Goat Anti-Rabbit IgG (H&L) - AcalephFluor680

Referência CSA4011-500ul

Tamanho : 500ul

Marca : Cohesion Biosciences

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  • Line colors represent the approximate visible colors of the wavelength maxima.
  • Western blot analysis of Beta-actin expression in Jurkat (A), MCF7 (B), NIH3T3 (C), mouse brain (D), mouse liver (E), rat brain (F), rat liver (G) whole cell lysates. Antibody binding was detected using Goat Anti-Rabbit IgG (H&L) - AcalephFluor680 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
Product Name:Goat Anti-Rabbit IgG (H&L) - AcalephFluor680
Cat No:CSA4011
Source:Goat
Reactivity:Rb
Applications:E, WB, IH, IF, FC
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Description:Goat Polyclonal Secondary Antibody to Rabbit IgG (H&L) - AcalephFluor680
Immunogen:Rabbit IgG
Purification:The antibody was isolated from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Specificity:By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG. No antibody was detected against non immunoglobulin serum proteins.
Clonality:Polyclonal
Conjugation:AcalephFluor680
Form:1 mg/ml. Liquid in 0.01M Phosphate Buffered Saline, pH 7.2, containing 1% BSA, 50% glycerol, 0.02% Sodium Azide
Dilution:WB (1/5000 - 1/20000), IH (1/100 - 1/1000), IF (1/100 - 1/1000), FC (1/1000 - 1/4000), E (Use at an assay dependent concentration)
Platform:Ex/Em = 680/701 nm
Storage/Stability:Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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