IgE (AP)

Referência I1900-19B-500ug

Tamanho : 500ug

Marca : US Biological

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I1900-19B IgE (AP)

Clone Type
Polyclonal
Host
goat
Source
human
Swiss Prot
P01854
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

Applications:
Suitable for use in ELISA and Western Blot. Other applications have not been tested.

Recommended Dilutions:
ELISA: 1:1000-1:10,000
Western Blot: 1:1000-1:5000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Applications
Product Type: Pab|Isotype: IgG|Host: goat|Source: human|Concentration: ~0.5mg/ml|Form: Supplied as a liquid in 50mM HEPES, 0.1M sodium chloride, 1mM magnesium chloride, 0.1mM ZnCl2, 0.2% BSA, 0.09% sodium azide. Labeled with alkaline phosphatase (AP).|Purity: Purified by affinity chromatography.|Immunogen: Native human IgE|Specificity: Recognizes human IgE. Species crossreactivity: Rhesus Monkey.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Native human IgE
Form
Supplied as a liquid in 50mM HEPES, 0.1M sodium chloride, 1mM magnesium chloride, 0.1mM ZnCl2, 0.2% BSA, 0.09% sodium azide. Labeled with alkaline phosphatase (AP).
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human IgE. Species crossreactivity: Rhesus Monkey. Minimal cross-reactivity (<0.1%) is expected with other human immunoglobulins and light chains.
References
1. Khan, F.M. et al. (2012) Basophil activation test compared to skin prick test and fluorescence enzyme immunoassay for aeroallergen-specific Immunoglobulin-E.|Allergy Asthma Clin Immunol. 8: 1. 2. Pinot de Moira, A. (2012) Childhood schistosome and hookworm co-infections: effects of treatment on IgE responses against parasite allergen-like proteins and immunity to reinfection. Infect Immun. Oct 15.