GDF15 Chemi-Luminescent ELISA Kit (Rat)
Referência OKCD03788
Tamanho : 96Wells
Marca : Aviva Systems Biology
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Telefone : +1 850 650 7790
GDF15 Chemi-Luminescent ELISA Kit (Rat) (OKCD03788)
| Datasheets/Manuals | Printable datasheet for GDF15 Chemi-Luminescent ELISA Kit (Rat) (OKCD03788) |
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| Predicted Species Reactivity | Rat|Rattus norvegicus | ||||||||||||||||||||||
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| Application | CLIA | ||||||||||||||||||||||
| ELISA Kit Detection Method | Chemiluminescent | ||||||||||||||||||||||
| ELISA Kit Duration | 2h, 40min | ||||||||||||||||||||||
| ELISA Kit Linearity |
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| ELISA Kit Principle | The microplate provided in this kit has been pre-coated with an antibody specific to Growth Differentiation Factor 15 (GDF15). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Growth Differentiation Factor 15 (GDF15). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Growth Differentiation Factor 15 (GDF15) level in the sample or standard. | ||||||||||||||||||||||
| ELISA Kit Range | 2.74-2,000pg/mL | ||||||||||||||||||||||
| ELISA Kit Recovery |
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| ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Differentiation Factor 15 (GDF15) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Differentiation Factor 15 (GDF15) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
| ELISA Kit Component |
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| Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
| Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 31.2 pg/mL | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | Gdf15 |
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| Gene Full Name | growth differentiation factor 15 |
| Alias Symbols | GDF-15;growth/differentiation factor 15. |
| NCBI Gene Id | 29455 |
| Protein Name | Growth/differentiation factor 15 |
| Description of Target | egulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses. Binds to its receptor, GFRAL, and activates GFRAL-expressing neurons localized in the area postrema and nucleus tractus solitarius of the brainstem. It then triggers the activation of neurons localized within the parabrachial nucleus and central amygdala, which contitutes part of the 'emergency circuit' that shapes feeding responses to stressful conditions (Probable). On hepatocytes, inhibits growth hormone signaling (By similarity). |
| Uniprot ID | Q9Z0J6 |
| Protein Accession # | NP_062089 |
| Nucleotide Accession # | NM_019216 |
