Fish thyroid stimulating hormone(TSH) ELISA Kit
Referência CSB-EQ027261FI-96T
Tamanho : 96T
Marca : Cusabio
Contactar o distribuidor local :
Telefone : +1 850 650 7790
| Size | 96T,5×96T,10×96T |
Product Details
Target Name
thyroid stimulating hormone(TSH)
Associated names
tshb ELISA Kit; Thyrotropin subunit beta ELISA Kit; Thyroid-stimulating hormone subunit beta ELISA Kit; TSH-B ELISA Kit; TSH-beta ELISA Kit; Thyrotropin beta chain ELISA Kit
Abbreviation
TSH
Uniprot No.
Species
Fish
Sample Types
serum, plasma, tissue homogenates
Detection Range
2.5 μIU/mL-40 μIU/mL
Sensitivity
2.5 μIU/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Competitive
Precision
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of fish TSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 100 | ||||
| Range % | 96-106 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 84-94 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 91-101 | |||||
Recovery
| The recovery of fish TSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 86-93 | ||||
| EDTA plasma (n=4) | 95 | 89-98 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Fish TSH ELISA Kit was designed for the quantitative measurement of Fish TSH protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 2.5 μIU/mL-40 μIU/mL and the sensitivity is 2.5 μIU/mL .
Citations
- P Iglesias-Hernández, MM Palencia,SSRN,2026
- N Korkmaz, K Erdoğan, GN Örün, B Erkmen,BMC Veterinary Research,2025
- Z Cai, G Zhou, X Yu, Y Du, Q Man, WC Wang,Ecotoxicology and Environmental Safety,2025
- Y Ka, I Lee, K Ji,Ecotoxicology and Environmental Safety,2024
- 박유진,서울대학교 대학원,2024
- 박소은,서울대학교 대학원,2024
- P Wei,Aquatic toxicology,2023
- Y Qiu,Ecotoxicology and environmental safety,2021
- S Lee,Toxics,2020
- Lei L,Chemosphere,2020
Customer Reviews and Q&A
There are currently no reviews for this product.
Target Background
Function
(From Uniprot)
(From Uniprot)
Indispensable for the control of thyroid structure and metabolism. May play some role in the biological processes of the immature fishes.
Subcellular Location
Secreted.
Protein Families
Glycoprotein hormones subunit beta family
Tissue Specificity
Pituitary gland. Higher levels seen in immature fishes than the mature fishes.
Database Links
UniGene: