Fish Prolactin/Luteotropic Hormone(PRL/LTH) ELISA Kit
Referência CSB-E12695Fh-5x96T
Tamanho : 5x96T
Marca : Cusabio
Contactar o distribuidor local :
Telefone : +1 850 650 7790
| Size | 96T,5×96T,10×96T |
Product Details
Target Name
Prolactin/Luteotropic Hormone(PRL/LTH)
Associated names
prl1 ELISA Kit; Prolactin-1 ELISA Kit; PRL-188 ELISA Kit; Prolactin I ELISA Kit
Abbreviation
PRL/LTH
Uniprot No.
Species
Fish
Sample Types
serum, plasma, tissue homogenates
Detection Range
10 μIU/mL-4000 μIU/mL
Sensitivity
10 μIU/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Competitive
Precision
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of fish PRL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 91-100 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 86-104 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-95 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 92-108 | |||||
Recovery
| The recovery of fish PRL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 91-102 | ||||
| EDTA plasma (n=4) | 95 | 88-105 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Fish PRL/LTH ELISA Kit was designed for the quantitative measurement of Fish PRL/LTH protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 10 μIU/mL-4000 μIU/mL and the sensitivity is 10 μIU/mL.
Citations
- G Rurale, I Gentile, L Persani, F Marelli,Journal of Molecular Medicine,2026
- M Villalba,Molecular Immunology,2020
- Bernard B.et al,Gen Comp Endocrinol,2018
- Na Na Kim et al,Marine and Freshwater Behaviour and Physiology,2013
Customer Reviews and Q&A
There are currently no reviews for this product.
■ Q&A
Q:
Which fish species / family is it optimized for? We work with a non-model species and would like to check its reactivity. Thank you.
A:
Thanks for your inquiry.
CSB-E12695Fh Fish Prolactin/Luteotropic Hormone(PRL/LTH) ELISA Kit
This kit is designed against salmon. There is no requirement for fish species. Gnerally all kinds of fish can be detected out. We suggest you do a pretest.
Pls let me know if you have any further questions. Thank you.
CSB-E12695Fh Fish Prolactin/Luteotropic Hormone(PRL/LTH) ELISA Kit
This kit is designed against salmon. There is no requirement for fish species. Gnerally all kinds of fish can be detected out. We suggest you do a pretest.
Pls let me know if you have any further questions. Thank you.
Target Background
Subcellular Location
Secreted.
Protein Families
Somatotropin/prolactin family
Database Links
UniGene: