Fish Interleukin 6,IL-6 ELISA Kit
Referência CSB-E13258Fh-96T
Tamanho : 96T
Marca : Cusabio
Contactar o distribuidor local :
Telefone : +1 850 650 7790
| Size | 96T,5×96T,10×96T |
Product Details
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
| To assess the linearity of the assay, samples were spiked with high concentrations of fish IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 88-97 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 82-94 | |||||
| 1:4 | Average % | 103 | ||||
| Range % | 94-107 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 90-101 | |||||
| The recovery of fish IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 100 | 91-104 | ||||
| EDTA plasma (n=4) | 91 | 84-98 | ||||
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||||||||||||||||||||||||||||||||||||||
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and FAQs
This Fish IL-6 ELISA Kit was designed for the quantitative measurement of Fish IL-6 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 12.5 pg/mL-200 pg/mL and the sensitivity is 12.5 pg/mL.
Citations
- M Abomosallam, A Ateya, AM Shosha, R Refaat,Aquaculture International,2025
- O Revina,/,2023
- Y Yan,Fish & shellfish immunology,2023
- NH Hassan,Pharmaceuticals,2023
- NO Erhunmwunse,researchsquare,2023
- NO Erhunmwunse,Research Square,2021
- Y Jia,Microbial pathogenesis,2021
- Yue Zhang,Fish & shellfish immunology,2020
- K Peng,Aquaculture Reports,2020
- I Ghosal,Aquaculture Research,2020
Customer Reviews and Q&A
Sample type: Serum Tissue homogenate Liver
Sample species: Fish
Sample dilution: No dilution
Review: I used CSB-E13258Fh to detect the serum of the normal group. The OD values of the standard品 were 2.594, 2.000, 1.209, 0.591, 0.342, and 0.193, and the OD values of the samples were between 0.6 and 1.7.
By Anonymous
Sample type: Serum
Sample species: Fish
Sample dilution: No dilution
Review: I used the CSB-E13258Fh enzyme-linked immunosorbent assay kit to determine Micropterus salmoides samples. The OD values of the standard curve are 2.429, 1.94, 1.174, 0.696, 0.337, and 0.204 respectively, and the sample OD values are between 0.6 and 1.6.
By Anonymous