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> Fish Insulin-like growth factor 1, IGF-1 ELISA Kit

Fish Insulin-like growth factor 1, IGF-1 ELISA Kit

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Referência CSB-E12122Fh-10x96T

Tamanho : 10x96T

Marca : Cusabio

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Telefone : +1 850 650 7790

CSB-E12122Fh
Size 96T,5×96T,10×96T

Product Details

Associated names
igf1Insulin-like growth factor I ELISA Kit; IGF-I ELISA Kit; Somatomedin ELISA Kit
Abbreviation
IGF-1
Uniprot No.
Species
Fish
Sample Types
serum, plasma, tissue homogenates
Detection Range
25 pg/mL-2000 pg/mL
Sensitivity
31.25 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of fish IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)  
1:1 Average % 94  
Range % 90-98  
1:2 Average % 95  
Range % 91-99  
1:4 Average % 102  
Range % 98-106  
1:8 Average % 87  
Range % 82-93  
Recovery
The recovery of fish IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 90-99  
EDTA plasma (n=4) 88 82-94  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average    
2000 0.341 0.343 0.342    
750 0.476 0.484 0.480    
375 0.682 0.636 0.659    
125 1.067 1.072 1.070    
25 1.488 1.567 1.528    
0 1.955 1.995 1.975    
ELISA Data Analysis
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description

This Fish IGF-1 ELISA Kit was designed for the quantitative measurement of Fish IGF-1 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 25 pg/mL-2000 pg/mL and the sensitivity is 31.25 pg/mL.

Citations

  • MM El-Naghy, SA Tawfik, MM Khalafalla, MM Zayed,Scientific Reports,2026
  • C Piyavorasakul, S Triwutanon,Journal of Fisheries and Environment,2025
  • AIG Elhetawy, MM Abdel-Rahim, SM Zeid,Veterinary Research Communications,2026
  • N Korkmaz, K Erdoğan, GN Örün, B Erkmen,BMC Veterinary Research,2025
  • TSM Saif, HH Mahmoud, AIG Elhetawy,Aquaculture International,2025
  • AIG Elhetawy, AT Mansour, AM Lotfy,Aquaculture International,2025
  • NA El-Fahla, H Abdelrazek, AS Fouad,Fish Physiology and Biochemistry,2025
  • AIG Elhetawy, MF El Basuini, AIA Mansour,BMC Veterinary Research,2025
  • JS Moon, M Seong, SC Kim, J Cadangin,Fisheries and Aquatic Sciences,2024
  • D Ay Güngör,Thesis&Dissertation,2024

Customer Reviews and Q&A

Average Rating:
5.0 - 1 reviews

Sample type: Serum

Sample species: Fish

Sample dilution: No dilution

Review: I used the CSB-E12122Fh kit to detect the IGF-1 hormone content in the serum of tilapia of different genders. The OD values are generally in the range of 0.2-0.8, and the effect is very good. This kit has simple steps, and the standard品 is already diluted, so there is no need to prepare it...

By Anonymous

■ Q&A
Q:

I want to use Fish Igf-1 ELISA kit for fish's liver homogenate.
In the protocol of the kit, for tissue homogenates, after homogenizing, stored overnight at -20oC. After 2 freeze-thaw cycles, centrifuge the homogenates.I do not understand too much about this. It means I have wait for 2 days to homogenate 2 sample (homogenate-> store -20oC-> thaw-> store 20oC -> thaw-> centrifuge)? Can you explain for me. Thanks!

A:
Thanks for your inquiry.Regarding tissue sample,pls store the sample which has been treated by PBS at -20 degrees overnight.After that,take the sample at room temperature and let it be thawed freely (the first freeze thaw cycle).Then freeze it at -80 degrees or store it at -20 degrees overnight.Then take it at temperature and let it be thawed freely.This is the 2nd freeze-thaw...
Q:

Can you let me know in which buffer the samples should be diluted?
This is not written in de manual!

A:
Thanks for your inquiry.
CSB-E12122Fh is no need to dilute generally.
So there is no sample diluent included in this kit.Pls let me know if you have any further questions. Thank you.
Q:

In the current protocol nothing is mentioned about an extraction of IGF-1 from binding proteins. This is generally a necessary procedure to properly measure IGF-1. Is an extraction, and if so which, recommended by Cusabio? Is this procedure in the most recent protocol?

A:
Thanks for your inquiry. There is no need to do extraction of IGF-1 from binding proteins for this kit.
Because this kit employs the competitive inhibition enzyme immunoassay technique and detects the total IGF1. Pls let me know if you have any further questions. Thank you.

Target Background

Function
(From Uniprot)
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are...
Gene References into Functions
  1. Nutrient availability, IGF-I, and genetic variation affect weight loss, in part through alterations of proteolytic pathways in rainbow trout.
Subcellular Location
Secreted.
Protein Families
Insulin family
Database Links

UniGene: