- A 96-well Assay plate --The 96-well plate has been pre-coated with anti-Fish GH antibody.
- Standard (6 x 1 ml) --Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
- HRP-avidin (1 x 6 ml) --Catalyze the TMB to elicit a chromogenic reaction.
- Biotin-conjugated GH (1 x 6 ml) --Bind to the precoated antibody and act as the detection antigen.
- Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration.
- Wash Buffer (20x concentrate) (1 x 15 ml) --Wash away unbound or free substances.
Fish growth hormone,GH ELISA Kit
Referência CSB-E12121Fh-10x96T
Tamanho : 10x96T
Marca : Cusabio
Contactar o distribuidor local :
Telefone : +1 850 650 7790
| Size | 96T,5×96T,10×96T |
Product Details
Target Name
growth hormone,GH
Associated names
gh1 ELISA Kit; Somatotropin-1 ELISA Kit; Growth hormone I ELISA Kit; Somatotropin I ELISA Kit
Abbreviation
GH
Uniprot No.
Species
Fish
Sample Types
serum, plasma, tissue homogenates
Detection Range
312.5 pg/mL-5000 pg/mL
Sensitivity
312.5 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Developmental Biology
Assay Principle
quantitative
Measurement
Competitive
Precision
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of fish GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 91-98 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 88-96 | |||||
| 1:4 | Average % | 102 | ||||
| Range % | 96-106 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 92-101 | |||||
Recovery
| The recovery of fish GH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 92-98 | ||||
| EDTA plasma (n=4) | 94 | 90-98 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||||||||||||||||||||||||||||||||||||||
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Materials provided
Materials not provided
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm.
- An incubator that can provide stable incubation conditions up to 37°C±5°
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Fish growth hormone (GH) ELISA Kit was designed for the quantitative measurement of Fish growth hormone (GH) protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 312.5 pg/mL-5000 pg/mL and the sensitivity is 312.5 pg/mL.
Citations
- MM El-Naghy, SA Tawfik, MM Khalafalla, MM Zayed,Scientific Reports,2026
- AM Valipour, B Heidari, S Ramezanpour,Aquaculture Research,2026
- GR Sallam, M Hamdy, MF El Basuini, SY El-Zaeem,Scientific Reports,2025
- M Tarek, S Rana, IA Himel, ZA Sunny,Aquaculture, Fish and Fisheries,2026
- AIG Elhetawy, MM Abdel-Rahim, SM Zeid,Veterinary Research Communications,2026
- Y Chae, YS Kwon, SW Kim, K Kim, YJ Kim,SSRN,/
- MC Hildebrand, D Runge, B Bassmann, HW Palm,Fishes,2025
- M Matinfar, B Heidari, AM Valipour, T Rahdari,PloS one,2025
- N Korkmaz, K Erdoğan, GN Örün, B Erkmen,BMC Veterinary Research,2025
- TSM Saif, HH Mahmoud, AIG Elhetawy,Aquaculture International,2025
Customer Reviews and Q&A
Average Rating:
Sample type: Serum
Sample species: Fish
Sample dilution: No dilution
Review: I used the CSB-E12121Fh kit to detect GH hormone levels in serum from tilapia of different genders, and the OD values were generally in the range of 0.2-0.8, with very good results. The kit has simple procedures, and the standard品 is already diluted, so there's no need to prepare it...
By Anonymous
Target Background
Function
(From Uniprot)
(From Uniprot)
Growth hormone plays an important role in growth control and is involved in the regulation of several anabolic processes. Implicated as an osmoregulatory substance important for seawater adaptation.
Subcellular Location
Secreted.
Protein Families
Somatotropin/prolactin family