Fish Cholecystokinin(CCK) ELISA Kit
Referência CSB-E13054Fh-96T
Tamanho : 96T
Marca : Cusabio
Contactar o distribuidor local :
Telefone : +1 850 650 7790
| Size | 96T,5×96T,10×96T |
Product Details
Target Name
Cholecystokinin(CCK)
Abbreviation
CCK
Uniprot No.
Species
Fish
Sample Types
serum, plasma, tissue homogenates
Detection Range
200 pg/mL-5000 pg/mL
Sensitivity
125 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Metabolism
Assay Principle
quantitative
Measurement
Competitive
Precision
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of fish CCK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 86-105 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 84-96 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 91-102 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-105 | |||||
Recovery
| The recovery of fish CCK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 85-91 | ||||
| EDTA plasma (n=4) | 95 | 86-104 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Fish CCK ELISA Kit was designed for the quantitative measurement of Fish CCK protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 200 pg/mL-5000 pg/mL and the sensitivity is 125 pg/mL.
Citations
- PG Ferreira, H Flávio,Journal of Experimental Biology,2026
- NA El-Fahla, H Abdelrazek, AS Fouad,Fish Physiology and Biochemistry,2025
- X Zhi, Y Gu, W Zhao, S Xie, Y Bao, S Morais, D Li,Aquaculture Reports,2025
- AS Bogevik,Aquaculture,2021
- Haokun Liu, et al,Aquaculture Research,2018
- Hamid Mohamadi Azarm et al,Aquaculture Research,2013
Customer Reviews and Q&A
There are currently no reviews for this product.
■ Q&A
Q:
Fish CCk ELISA kit cat.numb
Could you give me the specific size parameters of the plate wells? As she will need it for the plate reader machine.
A:
Thanks for your inquiry.
The kit size is 96T and it is 8wells× 12 strips.Pls check the size:
W：8.5cm
L：12.5cm
X：1.25cm
Y：1cm
Generally the microplate reader with 450nm wave can be used. Our company uses the reader from Thermo MK-3.Pls let me know if you have any further questions. Thank you.
The kit size is 96T and it is 8wells× 12 strips.Pls check the size:
W：8.5cm
L：12.5cm
X：1.25cm
Y：1cm
Generally the microplate reader with 450nm wave can be used. Our company uses the reader from Thermo MK-3.Pls let me know if you have any further questions. Thank you.