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- Product Name
- FineTest®700 Anti-Human CD273/PD-L2 Antibody(24F.10C12)
- Catalogue No.
- F700-30133
- Form
- liquid
- Conjugation
- FineTest®700
- Conjugation Information
- FineTest® 700 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 719 nm (e.g., a 725/40 nm bandpass filter).
- Clonality
- Monoclonal
- Isotype
- IgG2a, κ
- Clone ID
- 24F.10C12
- Storage
- PBS with 0. 1% sodium azide, 1%BSA, pH 7.3, 2-8℃ for 12 months (Avoid repeated freeze / thaw cycles.)
Immunogen
- Alternative Names
- B7DC|CD273|PDCD1L2|PDL2 antibody
- UniProt ID
- Q9BQ51
Application
- Tested Applications
- FC
- Recommended dilution
- Volume per test: 5μL. Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Validated Images
Staining of the CHO cells transfected with pcDNA3.1 plasmid encoding Human CD273/PD-L2 gene with FineTest®700 Anti-Human CD273/PD-L2 Antibody(24F.10C12) (filled gray histogram) or FineTest®700 Mouse IgG2a, κ Isotype Control(black histogram). Total viable cells were used for analysis. - Background
- CD273, known as B7-DC, is also called programmed death ligand 2 (PDL2). This ligand is a 25 kD type I transmembrane protein and a member of B7 family within the immunoglobulin receptor superfamily and is expressed on a subset of dendritic cells, liver and a small subset of macrophages as well as a few transformed cell lines. CD273 has been reported to be stimulatory on dendritic cells when cross-linked and to inhibit T cell activation upon engaging the PD-1 receptor. CD273 has also been reported to bind to an alternative receptor and to mediate T cell activation through such non-PD1 mediated interactions.