Ferroptosis Inhibitory Protein-1 (FSP-1) Activity Fluorometric Assay Kit

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Referência E-BC-F022-96T

Tamanho : 96T

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Telefone : +1 850 650 7790

Marca : Elabscience

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Contactar o distribuidor local :

Telefone : +1 850 650 7790

Ferroptosis Research Target Fluorescence microplate reader

Ferroptosis Suppressor Protein-1 (FSP-1) Activity Fluorometric Assay Kit (E-BC-F022)

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Manual MSDS

Ferroptosis Suppressor Protein-1 (FSP-1) Activity Fluorometric Assay Kit - 1

All	Size	Price	Qty
	96T	$ 520.00	- +

For research use only. Order now, ship in 3 days

Detection Principle	Ferroptosis inhibitory protein-1(FSP-1) induce non-caspase-dependent apoptosis based on factors such as C-terminal fragment of amino acid series, nuclear translocation and overexpression. Through the FSP1-CoQ10-NAD(P)H pathway and the classical glutathione (GSH)-GPX4 pathway, FSP-1 can protect cells from ferroptosis, and plays a "double-edged sword" role in cell life activities. The detection principle of this kit: FSP-1 catalyzes the substrate reaction to generate NADH, so that its fluorescence value increases under the excitation wavelength of 535 nm and emission wavelength of 587 nm. Adding inhibitors will inhibit the activity of FSP-1 and cause the rate of increase in fluorescence value to decrease, and the activity of FSP-1 can be calculated by measuring the difference value.
Detection Method	Fluorometric method
Sample Type	Animal and plant tissue;Cell
Assay Time	1 h
Detection Instrument	Fluorescence microplate reader
Ex/Em	Fluorescence microplate reader (Ex/Em=535 nm/587 nm)
Storage	This product can be stored at -20°C for 12 months with shading light.
Expiration date	12 months
Shipping	Ice bag

Cat.No.	Product Name	Sizes
E-BC-F101	Cell Ferrous Iron (Fe2+ ) Fluorometric Assay Kit	96T
E-BC-K880-M	Cell Total Iron Colorimetric Assay Kit	96T , 48T
E-BC-K881-M	Cell Ferrous Iron Colorimetric Assay Kit	96T , 48T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
10% Mouse liver tissue homogenate	2-5
10% Mouse heart tissue homogenate	1
10% Mouse kidney tissue homogenate	1-2
10% Mouse lung tissue homogenate	1
10% Mouse brain tissue homogenate	1
10% Green beans tissue homogenate	1
1×10^6 HL-60 cells	1
1×10^6 Hela cells	1

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.