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> FastPANGEA™ High Fidelity DNA Polymerase MasterMix

FastPANGEA™ High Fidelity DNA Polymerase MasterMix

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Referência P0061

Tamanho : 500rxn

Marca : Canvax Biotech

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Telefone : +1 850 650 7790

FastPangea High Fidelity DNA Polymerase MasterMix – Canvax

FastPANGEA™ High Fidelity DNA Polymerase MasterMix

FastPANGEA™ High Fidelity DNA Polymerase MasterMix is a second-generation, high-fidelity enzyme system delivering exceptional accuracy, speed, and yield for demanding PCR applications.
With an error rate 50× lower than standard Taq DNA Polymerase, it efficiently amplifies long or complex templates (up to 15 kb) while producing blunt-ended PCR products suitable for cloning.

FastPANGEA™ High Fidelity DNA Polymerase MasterMix represents the next generation of high-performance polymerases, engineered for maximum fidelity, speed, and robustness across a wide range of PCR applications.

This enzyme combines 5′→3′ DNA polymerase and 3′→5′ exonuclease (proofreading) activities, ensuring high-precision DNA synthesis with dramatically reduced error rates.
It consistently amplifies long targets (10–15 kb) and difficult GC-rich or repetitive regions with shorter extension times (15–30 s/kb) and minimal optimization.

The MasterMix format (2×) provides all necessary reaction components — polymerase, buffer, Mg²⁺, and dNTPs — in a convenient, ready-to-use formulation that enhances reproducibility and reduces pipetting errors.

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500 rxn

SKU: P0061 Categories: End Point PCR, MasterMixes

Detailed information:

Advantages & Features

  • Second-generation high-fidelity enzyme: improved formulation for maximal efficiency.
  • Extreme accuracy: ≥ 50× lower error rate than conventional Taq DNA Polymerase.
  • Robust amplification: reliable results across long or GC-rich templates.
  • Fast: extension rates of 15–30 s/kb, reducing total PCR time.
  • High yield: superior product recovery with minimal enzyme input.
  • Blunt-end products: suitable for blunt-end cloning or GC cloning.
  • Versatile: ideal for routine PCR, long-range amplification, and high-throughput workflows.

Specifications

Unit definition:
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP’s into acid-insoluble form in 30 minutes at 75 °C under assay conditions: 25 mM TAPS-HCl, pH 9.0 (at 25 °C), 100 mM KCl, 1.5 mM MgCl2, 1 mM Beta-mercaptoethanol, 200 μM each dNTP and 10 μg activated calf thymus DNA in 50 μl.

Includes

-4 x 1.25 mL FastPANGEA™ High Fidelity DNA Polymerase (2x)
– 500 μL DMSO (100%)
– 100 μL MgCl2 (25 mM)

*500 x  20 µl reactions
*200 x 50 µl reactions

 

Download documentation

Applications

  • PCR cloning and blunt-end ligation.
  • Primer extension and mutagenesis assays.
  • Long or difficult template amplification (10–15 kb).
  • High-throughput and multiplex PCR.
  • Sequencing library preparation and gene synthesis..

Tables & Figures

Quality Control

  • Functionally tested in PCR.
  • Undetected bacterial DNA (by PCR).

Advice

  • Note: Highly recommended for cloning into pSpark® DNA cloning vectors.

Storage, Shipping & Guarantee

  • Shipped in: Gel pack.
  • Storage: -20 °C.

Citations

  • CHAABENE, R. B. Role of associated rhoptry proteins in Toxoplasma gondii invasion.
  • Nyonda, M. A., Kloehn, J., Sosnowski, P., Krishnan, A., Lentini, G., Maco, B., … & Soldati-Favre, D. (2022). Ceramide biosynthesis is critical for establishment of the intracellular niche of Toxoplasma gondii. Cell reports, 40(7), 111224.
  • Lentini, Gaëlle, et al. “Structural insights into an atypical secretory pathway kinase crucial for Toxoplasma gondii invasion.” Nature Communications 12.1 (2021): 1-17.
  • Jiménez Muñoz, R. (2020). Desarrollo de herramientas fisiológicas y genómicas para mejorar la calidad postcosecha del fruto de calabacín.
  • García-Maceira, T., García-Maceira, F. I., González-Reyes, J. A., & Paz-Rojas, E. (2020). Highly enhanced ELISA sensitivity using acetylated chitosan surfaces.BMC Biotechnology, 20(1), 41.
  • Otero Bilbao, A. (2014). Characterization of two metallocarboxypeptidases of M14D subfamily from bacterial and human origin.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Customers Review

Synonym(s)

High fidelity DNA polymerase master mix, Proofreading DNA polymerase mix, High accuracy PCR master mix, PCR master mix high fidelity, DNA amplification master mix

Also known as:

  • Spanish: Master mix PCR de alta fidelidad, Polimerasa con proofreading, Mezcla PCR alta precisión
  • French: Master mix PCR haute fidélité, Polymérase avec activité proofreading, Mélange PCR haute précision
  • German: Hochfidele DNA-Polymerase Mastermix, PCR-Mix mit Proofreading, Hochpräzisions-PCR-Mix
  • Italian: Master mix PCR alta fedeltà, Polimerasi con proofreading, Miscela PCR alta precisione

FAQs

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331-50024-2
High-fidelity DNA polymerase
 500U