EIF2S1 Antibody - N-terminal region
Referência OAAB09763
Tamanho : 400µl
Marca : Aviva Systems Biology
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EIF2S1 Antibody - N-terminal region (OAAB09763)
| Datasheets/Manuals | Printable datasheet for EIF2S1 Antibody - N-terminal region (OAAB09763) |
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| Tested Species Reactivity | Mouse |
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| Predicted Species Reactivity | Bovine|Chicken|Human|Mouse|Porcine|Rat|Yeast|Zebrafish |
| Product Format | Liquid PBS with 0.09% (W/V) sodium azide. |
| Clonality | Polyclonal |
| Isotype | Ig |
| Host | Rabbit |
| Application | IF,IHC-P,WB |
| Additional Information | Cellular Location: Cytoplasmic granule. Note=The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Co-localizes with NANOS3 in the stress granules. |
| Reconstitution and Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Immunogen | This EIF2S1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 60-89 amino acids from the N-terminal region of human EIF2S1. |
| Purification | This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Concentration | 0.412 mg/ml |
| Application Info | WB: 1:1000 IF: 1:10~50 IHC-P: 1:10~50 |
| Gene Symbol | EIF2S1 |
|---|---|
| Gene Full Name | eukaryotic translation initiation factor 2 subunit alpha |
| Alias Symbols | 0910001O23Rik, 2410026C18Rik, 35kDa, CG9946, CG9946-PA, CG9946-PB, deIF2alpha, Dmel_CG9946, Dmel\CG9946, Eif, EIF-2, eIF-2 a, eIF-2 alpha, eIF-2-alpha, EIF-2A, EIF-2alpha, eIF-2alphaeIF-2alpha, EIF2, eIF2 alpha, eIF2 alpha subunit, eIF2-alpha, EIF2A, eIF2alpha, eIF2alpha-PA, eIF2alpha-PB, eif2s1, Elf, elF2alpha, eukaryotic initiation factor 2alpha, eukaryotic translation initiation factor 2, eukaryotic translation initiation factor 2 subunit 1, eukaryotic translation initiation factor 2 subunit 1 alpha, eukaryotic translation initiation factor 2 subunit 1b, eukaryotic translation initiation factor 2 subunit alpha, eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa, eukaryotic translation initiation factor 2A, eukaryotic translation initiation factor 2alpha, fb19d01, IF2A_DROME, l(1)14Cf, l(1)19-153, M(1)14C, M(1)19-153, M(1)8e3-10, minute (1) 14C, P-eIF2alpha, translation initiation factor eIF2 subunit alpha, wu:fb19d01, YJR007W. |
| NCBI Gene Id | 13665|1965|321564|32617|327694|423279|54318|853463 |
| Protein Name | Eukaryotic translation initiation factor 2 subunit 1|eukaryotic translation initiation factor 2 subunit 1 [Homo sapiens]|Eukaryotic translation initiation factor 2 subunit alpha|Eukaryotic translation initiation factor 2, subunit 1 alpha |
| Description of Target | eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.|eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This preinitiation complex mediates ribosomal recognition of a start codon during the scanning process of the leader region.|Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B (By similarity).|Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.|Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S pre-initiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. EIF2S1/eIF-2-alpha is a key component of the integrated stress response (ISR), required for adaptation to various stress: phosphorylation by metabolic-stress sensing protein kinases (EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2) in response to stress converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor, leading to a attenuation of cap-dependent translation, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activators ATF4 and QRICH1, and hence allowing ATF4- and QRICH1-mediated reprogramming. |
| Uniprot ID | P05198|P20459|P20460|P41374|P68101|P68102|Q5ZLX2|Q6ZWX6 |
| Protein Accession # | https://www.ncbi.nlm.nih.gov/protein/NP_004085.1 |
| Molecular Weight | 36112 |
