EF1, alpha (Elongation Factor 1a) (AP)

Referência MBS6241512-5x0,1mL

Tamanho : 5x0.1mL

Marca : MyBiosource

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EF1, alpha (Elongation Factor 1a) (AP)

EF1, Alpha (EEF1A1) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6241512

Human eukaryotic translation elongation factor 1 alpha has two isoforms, alpha 1 and alpha 2. The alpha 1 isoform gene encodes a subunit of the elongation factor-1 complex which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. The… More details

Product Overview

Online Datasheet in PDF
Product Name
EF1, alpha (EEF1A1), Monoclonal Antibody
Full Product Name
EF1, alpha (Elongation Factor 1a) (AP)
Product Gene Name
anti-EEF1A1 antibody
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
0.T.35
Host
Mouse
Species Reactivity
Mammal, Yeast
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Purity/Purification
Purified by Protein G Affinity Chromatography
Specificity
Recognizes EF-1a (Elongation factor-1a) at 53kD. 38kD protein occasionally detected in A431 cell lysate. Species Crossreactivity: Trypanosomes, yeast and mammals.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Molecular Weight
462
Chromosome Location
Chromosome: 6; NC_000006.11 (74225473..74230755, complement). Location: 6q14.1
OMIM
130590
3D Structure
ModBase 3D Structure for P68104
Immunogen
Crude calmodulin-binding proteins from Trypanosoma brucei isolated using calmodulin-affinity chromatography.
Conjugate
AP

Product Categories/Family for anti-EEF1A1 antibody

Antibodies; Proteins
Applications Tested/Suitable for anti-EEF1A1 antibody
Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-EEF1A1 antibody
IP: 4ug immunoprecipitated EF-1 a from an A431 RIPA cell lysate.
Applications are based on unconjugated antibody.
WB: 0.5-2ug/ml detects EF-1a in RIPA lysates from non-stimulated A431 cells. Cell lysate was resolved by electrophoresis, transferred to nitrocellulose, and probed with (0.5ug/ml). Proteins were visualized using an IgG (HRP) Gt xMo and a chemiluminescence detection system.

Western Blot (WB) of anti-EEF1A1 antibody

WB: 20 micrograms of Jurkat cell lysate was probed with EF1a (1ug/ml).
Mouse Anti-EEF1A1 Monoclonal Antibody – Cat# MBS6241512

Product References and Citations for anti-EEF1A1 antibody

1. Kimball SR, et al., (1999) J. Biol. Chem. 274:11647-11652. 2. Kaur K. and Ruben L. J., (1994) Biol Chem. 269:23045-23050. 3. Shang Y and Brown M., (2002) Science 295:2465-68. 4. Benkirane M. et al., (1998) J Biol Chem. 273:24898-905.

Other Notes

Small volumes of anti-EEF1A1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for EEF1A1 purchase

MBS6241512 is an antibody targeting EEF1A1. Also known as EF1, alpha (Elongation Factor 1a) (AP). Reactive in Mammal, Yeast. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for EEF1A1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
elongation factor 1-alpha 1
NCBI Official Synonym Full Names
eukaryotic translation elongation factor 1 alpha 1
NCBI Official Symbol
EEF1A1
NCBI Official Synonym Symbols
CCS3; EF1A; PTI1; CCS-3; EE1A1; EEF-1; EEF1A; EF-Tu; LENG7; eEF1A-1; GRAF-1EF; HNGC:16303
UniProt Protein Name
Elongation factor 1-alpha 1
UniProt Synonym Protein Names
Elongation factor Tu; EF-Tu; Eukaryotic elongation factor 1 A-1; eEF1A-1; Leukocyte receptor cluster member 7
UniProt Gene Name
EEF1A1
UniProt Synonym Gene Names
EEF1A; EF1A; LENG7; EF-1-alpha-1; EF-Tu; eEF1A-1
UniProt Entry Name
EF1A1_HUMAN
NCBI Protein Information
elongation factor 1-alpha 1; EF-1-alpha-1; EF1a-like protein; CTCL tumor antigen; elongation factor Tu; cervical cancer suppressor 3; elongation factor 1 alpha subunit; prostate tumor-inducing protein 1; eukaryotic elongation factor 1 A-1; leukocyte recep

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-EEF1A1 antibody

 Reference Product  PubMed Publications
 TPT1 antibody  >3 publications with EEF1A1 and TPT1
 RPL4 antibody  >2 publications with EEF1A1 and RPL4
 ZBTB16 antibody  >1 publications with EEF1A1 and ZBTB16
 EEF1B2 antibody  >1 publications with EEF1A1 and EEF1B2
 UBC antibody  >1 publications with EEF1A1 and UBC

Pathways associated with anti-EEF1A1 antibody

 Products by Pathway  Pathway Diagram
 EGFR1 Signaling Pathway antibodies  EGFR1 Signaling Pathway Diagram
 Eukaryotic Translation Elongation Pathway antibodies  Eukaryotic Translation Elongation Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Legionellosis Pathway antibodies  Legionellosis Pathway Diagram
 Legionellosis Pathway antibodies  Legionellosis Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Peptide Chain Elongation Pathway antibodies  Peptide Chain Elongation Pathway Diagram
 RNA Transport Pathway antibodies  RNA Transport Pathway Diagram
 RNA Transport Pathway antibodies  RNA Transport Pathway Diagram
 Translation Pathway antibodies  Translation Pathway Diagram

Diseases associated with anti-EEF1A1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >43 publications with EEF1A1 and Neoplasms
 Carcinoma Antibodies  >16 publications with EEF1A1 and Carcinoma
 Breast Neoplasms Antibodies  >9 publications with EEF1A1 and Breast Neoplasms
 Prostatic Neoplasms Antibodies  >9 publications with EEF1A1 and Prostatic Neoplasms
 Adenocarcinoma Antibodies  >7 publications with EEF1A1 and Adenocarcinoma
 Neoplasms, Experimental Antibodies  >3 publications with EEF1A1 and Neoplasms, Experimental
 Hypertrophy Antibodies  >3 publications with EEF1A1 and Hypertrophy
 Liver Neoplasms Antibodies  >3 publications with EEF1A1 and Liver Neoplasms
 Hyperplasia Antibodies  >3 publications with EEF1A1 and Hyperplasia
 Lung Neoplasms Antibodies  >3 publications with EEF1A1 and Lung Neoplasms