DOTAP chloride [132172-61-3]

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Referência NB-64-02329-50mg

Tamanho : 50mg

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Telefone : +1 850 650 7790

DOTAP chloride (Synonyms: 1,2-Dioleoyl-3-trimethylammonium-propane chloride)

Catalog No. T11086 Copy Product Info

Purity: 99.97%

DOTAP chloride (1, 2-dioleoyl-3-trimethylammonium-propane chloride) is an efficient cationic lipid, and its target is mainly the negatively charged components on the cell membrane. DOTAP chloride binds to negatively charged nucleic acids (such as DNA and RNA) by electrostatic interaction to form complexes, thereby achieving the delivery of nucleic acids.

DOTAP chloride

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Synonyms 1,2-Dioleoyl-3-trimethylammonium-propane chloride

Cas No. 132172-61-3

Batch Information

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Purity:99.97%

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COA HNMR LCMS

Product Introduction

DOTAP chloride AI Summary

DOTAP chloride exhibits varying levels of toxicity in different assays related to liver function. When complexed with specific compounds such as cholesterol or DOPE, it shows toxic effects on alanine aminotransferase (ALAT) and aspartate aminotransferase (ASAT) levels in mice, with toxicity levels ranging from 37.0 to 657.0. This indicates a significant impact on liver enzymes, suggesting a potential role in disrupting normal liver function and metabolism..

Note: Summary generated by AI. Data source: ChEMBL

Bioactivity

Description	DOTAP chloride (1, 2-dioleoyl-3-trimethylammonium-propane chloride) is an efficient cationic lipid, and its target is mainly the negatively charged components on the cell membrane. DOTAP chloride binds to negatively charged nucleic acids (such as DNA and RNA) by electrostatic interaction to form complexes, thereby achieving the delivery of nucleic acids.
Targets&IC50	293T cells:58.6 µM
In vitro	METHODS: Prostate cancer cell line PC3 and melanoma cell line M21 were treated with paclitaxel (PTX) loaded on DOTAP/DOPC liposome carriers (CLs) for 24 hours. The cytotoxicity was detected by measuring the absorbance at 490 nm using an enzyme-linked immunosorbent assay reader. RESULTS: With the increase of PTX content, the cytotoxic effect of DOTAP/DOPC CLs is enhanced. [1]
Synonyms	1,2-Dioleoyl-3-trimethylammonium-propane chloride

Chemical Properties

Molecular Weight	698.54
Formula	C₄₂H₈₀ClNO₄
Cas No.	132172-61-3
Smiles	C(OC(CCCCCCC/C=C\CCCCCCCC)=O)(COC(CCCCCCC/C=C\CCCCCCCC)=O)C[N+](C)(C)C.[Cl-]
Relative Density.	no data available

Storage & Solubility Information

Storage

Pure form: -20°C for 3 years | In solvent: -80°C for 1 year

Solubility Information

Ethanol: 130 mg/mL (186.1 mM), Sonication and heating are recommended.

DMSO: 125 mg/mL (178.94 mM), Sonication is recommended.

In Vivo Formulation

10% DMSO+40% PEG300+5% Tween 80+45% Saline: 4 mg/mL (5.73 mM), Sonication is recommended.

Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions.

Solution Preparation Table

DMSO/Ethanol

	1mg	5mg	10mg	50mg
1 mM	1.4316 mL	7.1578 mL	14.3156 mL	71.5779 mL
5 mM	0.2863 mL	1.4316 mL	2.8631 mL	14.3156 mL
10 mM	0.1432 mL	0.7158 mL	1.4316 mL	7.1578 mL
20 mM	0.0716 mL	0.3579 mL	0.7158 mL	3.5789 mL
50 mM	0.0286 mL	0.1432 mL	0.2863 mL	1.4316 mL
100 mM	0.0143 mL	0.0716 mL	0.1432 mL	0.7158 mL

Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density.