Dog thrombomodulin,TM ELISA Kit
Referência CSB-E06529c-96T
Tamanho : 96T
Marca : Cusabio
Contactar o distribuidor local :
Telefone : +1 850 650 7790
| Size | 96T,5×96T,10×96T |
Product Details
Target Name
thrombomodulin
Associated names
THBD ELISA Kit; Thrombomodulin ELISA Kit; TM ELISA Kit; CD antigen CD141 ELISA Kit
Abbreviation
Uniprot No.
Species
Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types
serum, plasma, tissue homogenates
Detection Range
23.44 pg/mL-1500 pg/mL
Sensitivity
5.86 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Competitive
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of dog TM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:10 | Average % | 97 | ||||
| Range % | 91-103 | |||||
| 1:20 | Average % | 94 | ||||
| Range % | 91-98 | |||||
| 1:40 | Average % | 90 | ||||
| Range % | 88-94 | |||||
| 1:80 | Average % | 93 | ||||
| Range % | 89-97 | |||||
Recovery
| The recovery of dog TM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 83-94 | ||||
| EDTA plasma (n=4) | 93 | 87-98 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Dog THBD ELISA Kit was designed for the quantitative measurement of Dog THBD protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 23.44 pg/mL-1500 pg/mL and the sensitivity is 5.86 pg/mL.
Customer Reviews and Q&A
There are currently no reviews for this product.
Target Background
Function
(From Uniprot)
(From Uniprot)
Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.
Gene References into Functions
- plasma levels are higher in dogs infected with Leishmania infantum
- cDNA sequence analysis, amino acid sequence homology, and tissue expression.
Subcellular Location
Membrane; Single-pass type I membrane protein.
Tissue Specificity
Expressed in lung, liver, spleen, kidney, pancreas and lymph node. Low expression in heart, cerebrum, urinary bladder and uterus.
Database Links
KEGG:
STRING:
UniGene: