Dog Complement 4(C4) ELISA kit
Referência CSB-EQ027970DO-24T
Tamanho : 24T
Marca : Cusabio
Contactar o distribuidor local :
Telefone : +1 850 650 7790
| Size | 96T,5×96T,10×96T |
Product Details
Target Name
Complement 4(C4)
Abbreviation
C4
Species
Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types
serum, plasma, tissue homogenates
Detection Range
31.25 ng/mL-2000 ng/mL
Sensitivity
7.8 ng/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Competitive
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of dog C4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 97 | ||||
| Range % | 92-105 | |||||
| 1:400 | Average % | 96 | ||||
| Range % | 92-99 | |||||
| 1:800 | Average % | 103 | ||||
| Range % | 98-109 | |||||
| 1:1600 | Average % | 87 | ||||
| Range % | 82-91 | |||||
Recovery
| The recovery of dog C4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 88-98 | ||||
| EDTA plasma (n=4) | 90 | 87-95 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Dog Complement 4 (C4) ELISA Kit was designed for the quantitative measurement of Dog Complement 4 (C4) protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 31.25 ng/mL-2000 ng/mL and the sensitivity is 7.8 ng/mL .
Citations
- J Zhou,Journal of veterinary internal medicine,2024
Customer Reviews and Q&A
Average Rating:
Sample type: Serum
Sample species: Dog
Sample dilution: : 1:9
Review: I used the canine complement C4 enzyme-linked immunosorbent assay kit to detect the C4 content in canine serum, and the results were good. The standard curve showed good linearity, the RSD of replicate wells was less than 15%, and the concentrations of diluted samples were all within the...
By Anonymous