Dog adrenomedullin,ADM ELISA Kit
Referência CSB-E09836c-24T
Tamanho : 24T
Marca : Cusabio
Contactar o distribuidor local :
Telefone : +1 850 650 7790
SDS| Size | 96T,5×96T,10×96T |
Product Details
Target Name
adrenomedullin
Associated names
ADM ELISA Kit; ADM [Cleaved into: Adrenomedullin ELISA Kit; AM); Proadrenomedullin N-20 terminal peptide ELISA Kit; ProAM N-terminal 20 peptide ELISA Kit; PAMP ELISA Kit; ProAM-N20)] ELISA Kit
Abbreviation
Uniprot No.
Species
Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types
serum, plasma, tissue homogenates, cell culture supernates
Detection Range
15.6 pg/mL-1000 pg/mL
Sensitivity
3.9 pg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of dog ADM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 87 | ||||
| Range % | 80-99 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-104 | |||||
| 1:4 | Average % | 98 | ||||
| Range % | 90-110 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 85-105 | |||||
Recovery
| The recovery of dog ADM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 82-104 | ||||
| EDTA plasma (n=4) | 97 | 90-110 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Dog ADM ELISA Kit was designed for the quantitative measurement of Dog ADM protein in serum, plasma, tissue homogenates, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL .
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Target Background
Function
(From Uniprot)
(From Uniprot)
AM and PAMP are potent hypotensive and vasodilatator agents.
Subcellular Location
Secreted.
Protein Families
Adrenomedullin family
Database Links
KEGG:
STRING:
UniGene: