Dnmt3l Antibody - middle region
Referência OAAB19087
Tamanho : 400ul
Marca : Aviva Systems Biology
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Dnmt3l Antibody - middle region (OAAB19087)
| Datasheets/Manuals | Printable datasheet for Dnmt3l Antibody - middle region (OAAB19087) |
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| Predicted Species Reactivity | Human|Mouse |
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| Product Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. |
| Clonality | Polyclonal |
| Isotype | Rabbit Ig |
| Host | Rabbit |
| Application | WB |
| Reconstitution and Storage | Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles. |
| Immunogen | This Mouse Dnmt3l antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 258-290 amino acids from the Central region of mouse Dnmt3l. |
| Purification | This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Application Info | WB: 1:1000 |
| Reference | Aapola U.,et al.Cytogenet. Cell Genet. 92:122-126(2001). Shaoping X.,et al.Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases. Carninci P.,et al.Science 309:1559-1563(2005). Bourc'his D.,et al.Science 294:2536-2539(2001). Gowher H.,et al.J. Biol. Chem. 280:13341-13348(2005). |
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| Gene Symbol | DNMT3L |
| Alias Symbols | D6Ertd14, D6Ertd14e, DNA (cytosine-5)-methyltransferase 3-like, ecat, ecat7. |
| NCBI Gene Id | 54427 |
| Protein Name | DNA (cytosine-5)-methyltransferase 3-like |
| Description of Target | Catalytically inactive regulatory factor of DNA methyltransferases that can either promote or inhibit DNA methylation depending on the context (PubMed:11719692, PubMed:15318244, PubMed:15671018, PubMed:24074865). Essential for the function of DNMT3A and DNMT3B: activates DNMT3A and DNMT3B by binding to their catalytic domain (PubMed:15671018). Acts by accelerating the binding of DNA and S-adenosyl-L-methionine (AdoMet) to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases (PubMed:15671018). Recognizes unmethylated histone H3 lysine 4 (H3K4me0) and induces de novo DNA methylation by recruitment or activation of DNMT3 (By similarity). Plays a key role in embryonic stem cells and germ cells (PubMed:11719692, PubMed:15318244, PubMed:24074865). In germ cells, required for the methylation of imprinted loci together with DNMT3A (PubMed:11719692). In male germ cells, specifically required to methylate retrotransposons, preventing their mobilization (PubMed:15318244). Plays a key role in embryonic stem cells (ESCs) by acting both as an positive and negative regulator of DNA methylation (PubMed:24074865). While it promotes DNA methylation of housekeeping genes together with DNMT3A and DNMT3B, it also acts as an inhibitor of DNA methylation at the promoter of bivalent genes (PubMed:24074865). Interacts with the EZH2 component of the PRC2/EED-EZH2 complex, preventing interaction of DNMT3A and DNMT3B with the PRC2/EED-EZH2 complex, leading to maintain low methylation levels at the promoters of bivalent genes (PubMed:24074865). Promotes differentiation of ESCs into primordial germ cells by inhibiting DNA methylation at the promoter of RHOX5, thereby activating its expression (PubMed:24074865). |
| Uniprot ID | Q9CWR8 |
| Molecular Weight | 47993 |
