DNASE1L3 ELISA Kit (Human)
Referência OKEH05157
Tamanho : 96Wells
Marca : Aviva Systems Biology
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DNASE1L3 ELISA Kit (Human) (OKEH05157)
| Datasheets/Manuals | Printable datasheet for DNASE1L3 ELISA Kit (Human) (OKEH05157) |
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| Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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| Application | ELISA-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~3 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology DNASE1L3 ELISA Kit (Human) (OKEH05157) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DNASE1L3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DNASE1L3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample DNASE1L3 captured in the well. | ||||||||||||||||||||||
| ELISA Kit Range | 0.312-20ng/mL | ||||||||||||||||||||||
| ELISA Kit Recovery | Mean recovery when spiking into Serum and Plasma = 93% | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision. Mean Intra-Assay Precision - < 8.5%, n = 20 Mean Inter-Assay Precision - < 11.2%, n = 20 | ||||||||||||||||||||||
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, plasma and other biological fluids | ||||||||||||||||||||||
| Sensitivity | 0.18 ng/mL | ||||||||||||||||||||||
| Specificity | Natural and recombinant Human Deoxyribonuclease gamma | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
| Gene Symbol | DNASE1L3 |
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| Gene Full Name | deoxyribonuclease 1 like 3 |
| Alias Symbols | deoxyribonuclease gamma, deoxyribonuclease I-like 3, deoxyribonuclease I-like III, DHP2, DNAS1L3, DNase gamma, DNase I homolog protein 2, DNase I homolog protein DHP2, DNase I-like 3, Liver and spleen DNase, LS-DNase, LSD, SLEB16. |
| NCBI Gene Id | 1776 |
| Protein Name | Deoxyribonuclease gamma |
| Description of Target | Has DNA hydrolytic activity. Is capable of both single- and double-stranded DNA cleavage, producing DNA fragments with 3'-OH ends (By similarity). Can cleave chromatin to nucleosomal units and cleaves nucleosomal and liposome-coated DNA (PubMed:9070308, PubMed:9714828, PubMed:14646506, PubMed:10807908, PubMed:27293190). Acts in internucleosomal DNA fragmentation (INDF) during apoptosis and necrosis (PubMed:23229555, PubMed:24312463). The role in apoptosis includes myogenic and neuronal differentiation, and BCR-mediated clonal deletion of self-reactive B cells (By similarity). Is active on chromatin in apoptotic cell-derived membrane-coated microparticles and thus suppresses anti-DNA autoimmunity (PubMed:27293190). Together with DNASE1, plays a key role in degrading neutrophil extracellular traps (NETs) (By similarity). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (By similarity). Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation (By similarity). |
| Uniprot ID | Q13609 |
| Protein Accession # | NP_001243489.1 |
| Nucleotide Accession # | NM_001256560.1 |
| Protein Size (# AA) | 305 |
