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Synonyms: Cytochalasin H2B
| Description | Dihydrocytochalasin B (H2CB) is a cell division inhibitor that prevents cytokinesis, alters cell morphology, disrupts actin structures in Swiss/3T3 mouse fibroblasts, and inhibits DNA synthesis induced by serum growth factors in quiescent cultures. Low doses of H2CB lead to cell rounding and the loss of actin microfilament bundles. |
| Targets & IC50 | P388/ADR cells:28 μM |
| In vitro | Dihydrocytochalasin B (2-10 X 10 (-7) M) disrupts the actin structure of Swiss/3T3 mouse fibroblasts and inhibits the ability of serum growth factor to stimulate DNA synthesis in static cultures. Low doses of H2CB cause cell rounding and loss of actin microfilaments, but they do not interfere with glucose or thymidine transport. [1] Dihydrocytochalasin B enhances reexpression of the phenotype of transformed growth factor-β induced differentiated chondrocytes without stimulating collagen synthesis. [2] Dihydrocytochalasin B (25 μg/mL) inhibited active calcium transport in intestinal absorption cells and led to increased Ca concentration in mucosal scrape. [3] The IC50 and IC80 of P388/ADR leukemia cell lines inhibited by Dihydrocytochalasin B were 28 μM and 48 μM, respectively. [4] |
| In vivo | Dihydrocytochalasin B (10 μM) is not toxic to zebrafish. Dihydrocytochalasin B is 20 times less toxic in mice than cytorelaxin D. [5] |
| Synonyms | Cytochalasin H2B |
| Molecular Weight | 481.62 |
| Formula | C29H39NO5 |
| Cas No. | 39156-67-7 |
| Smiles | O=C1[C@]23[C@]([C@H](CC4=CC=CC=C4)N1)([C@H](C)C(=C)[C@@H](O)[C@@]2(/C=C/C[C@H](C)CCC[C@@H](O)CCC(=O)O3)04)04 |
| Relative Density. | 1.194g/cm3 |
| Storage | Store at low temperature Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. | |||||||||||||||||||||||||
| Solubility Information | DMSO: 20 mg/mL (41.53 mM), Sonication is recommended. | |||||||||||||||||||||||||
Solution Preparation Table | ||||||||||||||||||||||||||
DMSO
Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density. | ||||||||||||||||||||||||||
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 µL Tween 80 and mix well until fully clarified.
3) Add 450 µL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
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