RayBio® Mouse Custom Adiponectin SpeedELISA is a sandwich-based immunoassay with a simplified work flow for the rapid, quantitative measurement of mouse Adiponectin in only 3 hours. Suitable for serum, plasma, cell culture supernatants, or lysate samples.
Custom Mouse Adiponectin SpeedELISA Kit
Custom Mouse Adiponectin SpeedELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Mouse |
| Accession Number | Q60994 |
| Gene Id | 11450 |
| Gene Symbols | ADIPOQ, ACRP30, ACDC, APM1 |
| Protein Name / Synonyms | Adiponectin (30 kDa adipocyte complement-related protein) (Adipocyte complement-related 30 kDa protein) (ACRP30) (Adipocyte, C1q and collagen domain-containing protein) (Adipose most abundant gene transcript 1 protein) (apM-1) (Gelatin-binding protein) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Cancer, Inflammation, Energy Metabolism, Obesity & Metabolic Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- Compressed workflow with only one wash step and three incubation steps
- 3 hours total processing time
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Application Notes
Kit Components
- Streptavidin-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Capture Antibody
- HRP Conjugated Detection Antibody
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 70 µl of the Antibody Cocktail to each well.
- Incubate 10 minutes at RT.
- Add 50 µl of sample or standard to each well.
- Incubate 2 h at RT.
- Wash Wells
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
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Excel (Sandwich-ELISA-Analysis-Tool.xlsm)
