For general laboratory use.

Please centrifuge briefly before opening (volume ≤2 ml).

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term storage (up to 3 month) at 4 °C possible.

Shelf Life: 12 months

Form: liquid

Concentration: 2x conc.

Description:
Crystal PCR Master is a 2 x conc. ready-to-use master mix recommended for routine PCR applications (up to 4 kb fragment length), high throughput PCR or genotyping.
It contains all reagents required for PCR (except template and primer) in a well-balanced ratio to ensure high specificity and minimal by-product formation in almost all PCR applications without the need of additional optimization steps.
The mix guarantees robust and reliable amplification results with a minimum of pipetting steps, saves time and reduces the risk of contaminations.
The total PCR assay volume is freely adaptable to individual protocols or the requirements of automated pipetting systems.

Content:

2 x concentrated PCR master mix containing Taq polymerase, nucleotides (dATP, dCTP, dGTP, dTTP), KCl, (NH₄)₂SO₄, MgCl₂, density reagent, enhancing and stabilizing additives.

Recommended PCR assay:
Before starting, vortex the master mix thoroughly to assure homogeneity.

Recommended cycling conditions:
Before cycling, vortex PCR tubes or plates to assure homogeneity and centrifuge briefly to remove bubbles.

¹⁾The annealing temperature depends on the melting temperature of the primers used.
²⁾The elongation time depends on the length of the fragments to be amplified. A time of 1 min/kb is recommended.

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