Creatine is a nitrogenous organic acid and is naturally present in vertebrates. Creatine maintains a high ATP/ADP ratio and helps to supply energy to cells in the body, especially muscle. Creatine supplementation is used as a sport performance enhancer and to treat muscular, neurological and neurodegenerative diseases. Detection of creatine level has wide significance in research and development.

 The Creatine Colorimetric/Fluorometric Assay Kit provides a sensitive, fast and convenient way for accurate detection of creatine levels in various biological fluids based on colorimetric and fluorometric method. In the assay, creatine is converted to sarcosine by creatinase. Sarcosine is then specifically oxidized to yield a product that converts a colorless probe to a highly fluorescent (Ex/Em = 538/587 nm) and intensely red color (λmax = 570 nm) product. The kit can detect  0.001-10 mM creatine.