Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded cow lactatingreacted with CPT1A polyclonal antibody (Cat # PAB4370), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western Blot (Cell lysate)
Western blot analysis of CPT1A polyclonal antibody (Cat # PAB4370) in Y-79 cell line lysate (35 ug/lane). CPT1A (arrow) was detected using the purified CPT1A polyclonal antibody (Cat # PAB4370).
- Specifications
Product Description
Rabbit polyclonal antibody raised against synthetic peptide of CPT1A.
Immunogen
A synthetic peptide (conjugated with KLH) corresponding to C-terminus of human CPT1A.
Host
Rabbit
Reactivity
Bovine, Human
Form
Liquid
Purification
Protein G purification
Recommend Usage
ELISA (1:1000)
Western Blot (1:100-500)
Immunohistochemistry (1:50-100)
The optimal working dilution should be determined by the end user.Storage Buffer
In PBS (0.09% sodium azide)
Storage Instruction
Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.Note
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Applications
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Formalin-fixed and paraffin-embedded cow lactatingreacted with CPT1A polyclonal antibody (Cat # PAB4370), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.
This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Enzyme-linked Immunoabsorbent Assay
Western Blot (Cell lysate)
Western blot analysis of CPT1A polyclonal antibody (Cat # PAB4370) in Y-79 cell line lysate (35 ug/lane). CPT1A (arrow) was detected using the purified CPT1A polyclonal antibody (Cat # PAB4370). - Gene Info — CPT1A
Entrez GeneID
1374Protein Accession#
CPT1_HUMANGene Name
CPT1A
Gene Alias
CPT1, CPT1-L, L-CPT1
Gene Description
carnitine palmitoyltransferase 1A (liver)
Gene Ontology
HyperlinkGene Summary
The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
carnitine palmitoyltransferase 1A|carnitine palmitoyltransferase I, liver
- Interactomes
- Pathways
- Diseases
- Publication Reference
- Malonyl coenzyme A and the regulation of functional carnitine palmitoyltransferase-1 activity and fat oxidation in human skeletal muscle.
Rasmussen BB, Holmback UC, Volpi E, Morio-Liondore B, Paddon-Jones D, Wolfe RR.
The Journal of Clinical Investigation 2002 Dec; 110(11):1687.
- Expression analysis of two mutations in carnitine palmitoyltransferase IA deficiency.
Ogawa E, Kanazawa M, Yamamoto S, Ohtsuka S, Ogawa A, Ohtake A, Takayanagi M, Kohno Y.
Journal of Human Genetics 2002 Jan; 47(7):342.
Application：WB-Tr, Human, Human fibroblasts.
- Malonyl coenzyme A and the regulation of functional carnitine palmitoyltransferase-1 activity and fat oxidation in human skeletal muscle.