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> Connexin 30

Connexin 30

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Referência C7853-61-50ug

Tamanho : 50ug

Marca : US Biological

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C7853-61 Connexin 30

Clone Type
Polyclonal
Host
rabbit
Source
mouse
Swiss Prot
P70689
Isotype
IgG
Grade
Affinity Purified
Applications
E IC IF IHC WB
Crossreactivity
Fe Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

The connexin gene family codes for the protein subunits of gap junction channels, which mediate the direct diffusion of ions and metabolites between the cytoplasm of adjacent cells. The gap junction channels have unique properties depending on the type of connexins constituting the channel.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence/Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1-3ug/ml
Immunohistochemistry: 1-5ug/ml
Immunohistochemistry (Paraffin): 1:20-1:200 Requires HIER using sodium citrate buffer pH6.0 prior to staining.
Immunohistochemistry (Frozen): 1-5ug/ml
Immunofluorescence (IC): 1:100-1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Pab|Isotype: IgG|Host: rabbit|Source: mouse|Concentration: ~0.1mg/ml|Form: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide|Purity: Purified by immunoaffinity chromatography|Immunogen: Synthetic peptide corresponding to the unique C-terminus of the mouse Connexin 30 protein. Species sequence homology: chicken, guinea pig|Specificity: Recognizes mouse Connexin 30. Crossreactivity with the highly related Connexin 26 protein or with other Connexin family members has not been detected. Species Crossreactivity: human, feline and rat||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Synthetic peptide corresponding to the unique C-terminus of the mouse Connexin 30 protein. Species sequence homology: chicken, guinea pig
Form
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide
Purity
Purified by immunoaffinity chromatography
Specificity
Recognizes mouse Connexin 30. Crossreactivity with the highly related Connexin 26 protein or with other Connexin family members has not been detected. Species Crossreactivity: human, feline and rat
References
1. Saez, J.C., et al., Advances in Second Messenger and Phosphorylation Research (1993). 2. Bennet, M.V.L., et al., Neuron 6: 305-320 (1990). 3. Beyer, E., et al., J. Cell. Biol. 105: 2621-2629 (1987). 4. Beyer, E.C., J. Cell. Biol. 108: 595-605 (1989). 5. Crow, D.S., et al., Mol. Cell. Biol. 10: 1754-1763 (1990). 6. Fishman, G.I., et al., J. Cell. Biol. 111: 589-598 (1990). 7. Kanter, H.L., et al., Circ. Res. 73: 344-350 (1993). 8. Wilgenbus, K.K., et al., Int. J. Cancer 51: 522-529 (1992). 9. Davis, L.M., et al., J. Amer. Coll. Cardiol. 24: 1124-1132 (1994). 10. Dahl, E., et al., J. Biol. Chem. 271: 17,903-17,910 (1996). 11. Nagy, J.I., et al., Neuroscience 78: 533-548 (1997). 12. Rash, J.E., et al., J. Neuroscience 21(6): 1983-2000 (2001). (Also used for freeze-fracture immunogold labeling for double labeling and electron microscopy). 13. Nagy, J.I., et al., Neuroscience 88(2): 447-468 (1998).