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> Collagen Hybridizing Peptide, Cy3 Conjugate

Collagen Hybridizing Peptide, Cy3 Conjugate

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Description
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Description

The collagen hybridizing peptide (CHP) is a novel and unique peptide that specifically binds unfolded collagen chains, both in vitro and in vivo.[1,2,3] By sharing the Gly-X-Y repeating sequence of natural collagen, CHP has a strong capability to hybridize with denatured collagen chains by reforming the triple helical structure, in a fashion similar to DNA fragments annealing to complementary DNA strands. CHP is extremely specific: it has negligible affinity to intact collagen molecules due to lack of binding sites, and it is inert towards non-specific binding because of its neutral and hydrophilic nature.

CHP is a powerful histopathology tool which enables straightforward detection of inflammation and tissue damage caused by a large variety of diseases, as well as tissue remodeling during development and aging.[3] CHP robustly visualizes the pericellular matrix turnover caused by proteolytic migration of cancer cells within 3D collagen culture, without the use of synthetic fluorogenic matrices or genetically modified cells.[4] CHP can measure and localize mechanical injury to collagenous tissue at the molecular level.[5] It also enables assessment of collagen denaturation in decellularized extracellular matrix.[6] In addition, CHP can be used to specifically visualize collagen bands in SDS-PAGE gels without the need for western blot.[7]

R-CHP is labeled with sulfo-Cyanine3 for direct fluorescence detection.

Specificity: CHP binds to the unfolded triple-helical chains of all collagen types (e.g., I, II, III, IV, etc).[3,7]

Applications: immunofluorescence,[3] cell imaging,[4] SDS-PAGE (in-gel western)[7]

Specification

Synonyms R-CHP, collagen mimetic peptide (CMP)
Molecular weight 3191.44 g/mol
Purity 85% by HPLC
Conjugate Single sulfo-Cyanine3 tag per peptide
Excitation 548 nm
Emission 563 nm
Content Purified lyophilized powder
Storage -20 °C as powder, 4 °C after reconstitution in water

 

Features

  • rmative, reliable and convenient than zymography, DQ collagen, SHG, and TEM
  • High affinity and unparalleled specificity to collagen with essentially no nonspecific binding
  • Applicable to all types of collagen from all species, relying on collagen's secondary structure instead of any defined sequence for binding
  • Suitable for both frozen and paraffin-embedded sections with no need for antigen retrieval
  • A non-antibody approach with no species restrictions against any co-staining antibody
  • Small size (2% of IgG by MW) enabling facile tissue penetration and whole specimen staining without sectioning
  • Stable in solution under 4 °C, eliminating the need to aliquot for storage 

For research use only. Not intended or approved for diagnostic or therapeutic use.

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Selected Citations
Selected Citations
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  • Fei, Y., et al. (2024). Promoting Chondrogenesis by Targeted Delivery to the Degenerating Cartilage in Early Treatment of Osteoarthritis. Bioactive Materials, 40, 624–633. doi: 10.1016/j.bioactmat.2024.08.004
  • Fang, Y., et al. (2024). Collagen Denaturation in Post-Run Achilles Tendons and Achilles Tendinopathy: In Vivo Mechanophysiology and Magnetic Resonance Imaging. Science Advances, 10(40), eado2015. doi: 10.1126/sciadv.ado2015
  • Rappold, R., et al. (2024). Salmonella‐Driven Intestinal Edema in Mice is Characterized by Tensed Fibronectin Fibers. The FEBS Journal. Advance online publication. doi: 10.1111/febs.17190
  • Dhiman, M. S., et al. (2024). Collagen Integrity of the Annulus Fibrosus in Degenerative Disc Disease Individuals Quantified with Collagen Hybridizing Peptide. JOR Spine, 7(3), e1359. doi: 10.1002/jsp2.1359
  • Satoh, A., et al. (2024). Keratinocyte-Driven Dermal Collagen Formation in the Axolotl Skin. Developmental Biology, 509, 56–66. doi: 10.1016/j.ydbio.2024.03.003
  • Zhao, J., et al. (2024). Delineating, Imaging, and Assessing Pulmonary Fibrosis Remodeling via Collagen Hybridization. ACS Nano, 18(41), 27997–28011. doi: 10.1021/acsnano.4c06139
  • Ahmad, A., et al. (2024). Molecular Imaging of Collagen Remodeling in a Murine Model of Lung Fibrosis. bioRxiv. Preprint. doi: 10.1101/2024.03.01.582999
  • Wang, H., et al. (2024). Chiral Arginine Modified Electrospun Membrane for Enhancing Tendon Healing. Advanced Functional Materials, 2402845. doi: 10.1002/adfm.202402845
  • Xiao, Z., et al. (2023). Desmoplastic Stroma Restricts T Cell Extravasation and Mediates Immune Exclusion and Immunosuppression in Solid Tumors. bioRxiv. Preprint. doi: 10.1101/2023.04.13.536777
  • Loflin, B. E., et al. (2023). An Adolescent Murine In Vivo Anterior Cruciate Ligament Overuse Injury Model. The American Journal of Sports Medicine, 51(7), 1721–1732. doi: 10.1177/03635465231165753
  • Smith, K. A., et al. (2023). Collagen Molecular Damage is a Hallmark of Early Atherosclerosis Development. Journal of Cardiovascular Translational Research, 16(2), 463–472. doi: 10.1007/s12265-022-10316-y
  • Salarian, M., et al. (2023). Homeostatic, Non-Canonical Role of Macrophage Elastase in Vascular Integrity. Circulation Research, 132(4), 432–448. doi: 10.1161/CIRCRESAHA.122.322096
  • Flinn, M. A., et al. (2023). Myofibroblast Ccn3 is Regulated by Yap and Wwtr1 and Contributes to Adverse Cardiac Outcomes. Frontiers in Cardiovascular Medicine, 10, 1142612. doi: 10.3389/fcvm.2023.1142612
  • Lim, P. J., et al. (2023). Perturbations in Fatty Acid Metabolism and Collagen Production Infer Pathogenicity of a Novel MBTPS2 Variant in Osteogenesis Imperfecta. Frontiers in Endocrinology, 14, 1195704. doi: 10.3389/fendo.2023.1195704
  • Ho, Y.-C., et al. (2023). PROX1 Inhibits PDGF-B Expression to Prevent Myxomatous Degeneration of Heart Valves. Circulation Research, 133(6), 463–480. doi: 10.1161/CIRCRESAHA.123.323027
  • Nishi, K., et al. (2023). A Thioacetamide-Induced Liver Fibrosis Model for Pre-Clinical Studies in Microminipig. Scientific Reports, 13(1), 14996. doi: 10.1038/s41598-023-42144-8
  • Zhang, Y., et al. (2023). Omentin-1 Induces Mechanically Activated Fibroblasts Lipogenic Differentiation through PKM2/YAP/PPARγ Pathway to Promote Lung Fibrosis Resolution. Cellular and Molecular Life Sciences, 80(10), 308. doi: 10.1007/s00018-023-04961-y
  • Bui, T. V. A., et al. (2023). Core-Shell Droplet-Based Angiogenic Patches for the Treatment of Ischemic Diseases: Ultrafast Processability, Physical Tunability, and Controlled Delivery of an Angiogenic Cocktail. ACS Applied Materials & Interfaces, 15(44), 50693–50707. doi: 10.1021/acsami.3c09062
  • Sun, A. R., et al. (2023). Hybrid Hydrogel-Extracellular Matrix Scaffolds Identify Distinct Ligand and Mechanical Signatures in Cardiac Aging. bioRxiv. Preprint. doi: 10.1101/2023.10.11.561941
  • Jones, B., et al. (2022). Structurally Abnormal Collagen Fibrils in Abdominal Aortic Aneurysm Resist Platelet Adhesion. Journal of Thrombosis and Haemostasis, 20(2), 470–477. doi: 10.1111/jth.15576
  • Smith, K. A. (2022). Evaluation of Hemodynamic Induced Alterations in Extracellular Matrix Organization in Atherogenesis [Doctoral dissertation, The University of Utah]. University of Utah Research Repository. Link to dissertation
  • Atta, G. (2022). The Lymphogenic and Hemangiogenic Factors Affecting the Human Sclera [Doctoral dissertation, Universität zu Köln]. Kölner UniversitätsPublikationsServer. Link to dissertation
  • Kim, M., et al. (2022). A New Murine Liver Fibrosis Model Induced by Polyhexamethylene Guanidine-Phosphate. Biomolecules & Therapeutics, 30(2), 126–133. doi: 10.4062/biomolther.2021.139
R-CHP | Collagen Hybridizing Peptide, Cy3 Conjugate

R-CHP | Collagen Hybridizing Peptide, Cy3 Conjugate

Collagen Hybridizing Peptide, Cy3 Conjugate (R-CHP), Product Vial
Collagen Hybridizing Peptide Stain: R-CHP Histology Stain
Collagen Hybridizing Peptide Stain: R-CHP Histology Stain
R-CHP | Collagen Hybridizing Peptide, Cy3 Conjugate

R-CHP | Collagen Hybridizing Peptide, Cy3 Conjugate

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