Clean-Easy™ PCR Purification Kit
Referência AN0064-XL
Tamanho : 250rxn
Marca : Canvax Biotech
Contactar o distribuidor local :
Telefone : +1 850 650 7790
Clean-Easy™ PCR Purification Kit
Rapid and efficient PCR product clean-up with up to 95% recovery in ~14 minutes.
The CleanEasy™ PCR Purification Kit provides a fast and efficient method for removing unwanted primers, nucleotides, salts and enzymes from PCR reactions. Using Canvax’s innovative HigherPurity™ silica technology, the kit delivers DNA of exceptional quality ready for direct use in sequencing, cloning and other downstream molecular biology applications.
Its simple spin-column workflow minimizes handling steps and ensures reliable, reproducible performance, making it the ideal choice for routine PCR product clean-up in any research laboratory.
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Detailed information:
Advantages & Features
- Really fast and easy procedure: it takes 14 minutes to get results with minimal handling steps.
- Reproducible yields: high DNA Recovery (70-90%) and consistent yields of pure DNA.
- Sensitive: proven performance for DNA fragments as short as 75 bp and up to 10 kb.
- Efficient removal of primers, nucleotides, salts and enzymes.
- Pure genomic DNA: ready-to-use in all Molecular Biology applications, including sequencing and cloning
Specifications
Includes
Includes for 50 rxn:
– 50 CleanEasy™ MiniSpin Columns
– 50 Collection tubes
– 25 mL PB Buffer
– 11.25 mL PE Buffer
– 9 mL EB Buffer
Download documentation
Applications
- Removal of proteins and salts from PCR, restriction digestion, dephosphorylation, ligation or labelling reactions.
- Changing of a restriction enzyme buffer.
- Sequencing.
- Re-purification of genomic DNA.
- In vitro transcription
Tables & Figures
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Quality Control
- Tested in the purification of a 0.6 kb DNA fragment from PCR Mixture.
- Purified band is analysed in Agarose Gel Electrophoresis.
Advice
- Avoid exceeding recommended DNA amounts per column.
- For maximum yield, prewarm elution buffer to 65 °C before use.
- Always equilibrate buffers to room temperature.
Storage, Shipping & Guarantee
- Shipped in: Ambient Temperature.
- Storage: Room Temperature.
Citations
- Morales-Hidalgo, M., Povedano-Priego, C., Martinez-Moreno, M. F., Ojeda, J. J., Jroundi, F., & Merroun, M. L. (2024). Long-term tracking of the microbiology of uranium-amended water-saturated bentonite microcosms: a mechanistic characterization of U speciation. Journal of Hazardous Materials, 135044.
- Lasheras Niese, V. (2022). Paparres presents en la fauna salvatge de Catalunya: identificació morfològica i verificació molecular d’espècies.
- Delgado Romero, José A. “Microorganismos extremófilos. Vida microbiana más allá de sus condiciones óptimas.” (2021).
- Robledo Mahón, T. (2018). Estudio de los procesos biológicos y de la estructura de las comunidades microbianas en el proceso de compostaje de lodos de depuradora de aguas residuales urbanas, en sistemas de membranas semipermea.
- Rodriguez-Sanchez, A., Margareto, A., Robledo-Mahon, T., Aranda, E., Diaz-Cruz, S., Gonzalez-Lopez, J., … & Gonzalez-Martinez, A. (2017). Performance and bacterial community structure of a granular autotrophic nitrogen removal bioreactor amended with high antibiotic concentrations. Chemical Engineering Journal, 325, 257-269.
- Vasta, V., Yáñez-Ruiz, D. R., Mele, M., Serra, A., Luciano, G., Lanza, M., … & Priolo, A. (2010). Bacterial and protozoal communities and fatty acid profile in the rumen of sheep fed a diet containing added tannins. Applied and Environmental Microbiology, 76(8), 2549-2555.
- Valverde-Tercedor, C., Abadía-Molina, F., Martinez-Bueno, M., Pineda-Molina, E., Chen, L., Oestreicher, Z., … & Jimenez-Lopez, C. (2014). Subcellular localization of the magnetosome protein MamC in the marine magnetotactic bacterium Magnetococcus marinus strain MC-1 using immunoelectron microscopy. Archives of microbiology, 196(7), 481-488.
- López-Fernández, M., Fernández-Sanfrancisco, O., Moreno-García, A., Martín-Sánchez, I., Sánchez-Castro, I., & Merroun, M. L. (2014). Microbial communities in bentonite formations and their interactions with uranium. Applied Geochemistry, 49, 77-86.
- Avilés, C., Juárez, M., Azor, P. J., Pajuelo, P., Polvillo, O., Álvarez, F., … & Spain, S. (2007). Association of single nucleotide polymorphisms in the µ-Calpain and Calpastatin gene with proteases activities in Retinta beef cattle: Preliminary results. In 53rd Internat. Congress of Meat Science and Technology, Beijing, China.
- Nudelman, H., Valverde-Tercedor, C., Kolusheva, S., Gonzalez, T. P., Widdrat, M., Grimberg, N., … & Jimenez-Lopez, C. (2016). Structure–function studies of the magnetite-biomineralizing magnetosome-associated protein MamC. Journal of structural biology, 194(3), 244-252.
- Moreno, B., Vivas, A., Nogales, R., & Benitez, E. (2009). Solvent tolerance acquired by Brevibacillus brevis during an olive-waste vermicomposting process. Ecotoxicology and environmental safety, 72(8), 2109-2114.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
Synonym(s)
PCR purification kit, DNA cleanup PCR kit, PCR cleanup kit silica column, DNA purification PCR products, PCR product purification kit
Also known as:
- Spanish: Kit de purificación de PCR, Limpieza de productos de PCR, Purificación de ADN PCR
- French: Kit de purification PCR, Nettoyage produits PCR, Purification ADN PCR
- German: PCR-Reinigungskit, Aufreinigung von PCR-Produkten, DNA-Reinigung PCR
- Italian: Kit purificazione PCR, Pulizia prodotti PCR, Purificazione DNA PCR
