Chicken Interferon Gamma (IFNg) ELISA Kit
Referência MBS2020832-96
Tamanho : 96-Strip-Wells
Marca : MyBiosource
Contactar o distribuidor local :
Telefone : +1 850 650 7790
Interferon Gamma (IFNg) ELISA Kit for Chicken
For Research Use Only. Not for use in diagnostic procedures. Special Promotion Popular Catalog #: MBS2020832SPECIAL PROMOTION: Get FREE Starbucks gift card ( for 48-Wells-Kit or for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to IFNg. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to IFNg. Next, Avidin… More details
Product Overview
Online Datasheet in PDFProduct Name
Interferon Gamma (IFNg), ELISA Kit
Full Product Name
Chicken Interferon Gamma (IFNg) ELISA Kit
Product Gene Name
IFNg elisa kit
Product Synonym Names
IFN-G; IFG; IFI; INFr; IFN, Immune Interferon
Species Reactivity
Chicken
Assay Type
Quantitative Sandwich
Samples
Gallus serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
15.6-1,000pg/mL
Sensitivity
< 5.5pg/mL
Specificity
This assay has high sensitivity and excellent specificity for detection of IFNg. No significant cross-reactivity or interference between IFNg and analogues was observed.
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IFNg were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IFNg were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Molecular Weight
19,348 Da
OMIM
147570
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Product Categories/Family for IFNg elisa kit
Cytokine; Infection immunity
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Other Notes
Small volumes of IFNg elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for IFNg purchase
MBS2020832 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Interferon Gamma (IFNg) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IFNg. The ELISA analytical biochemical technique of the MBS2020832 kit is based on IFNg antibody-IFNg antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IFNg antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IFNg. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Additional Details
NCBI/Uniprot data below describe general gene information for IFNg. It may not necessarily be applicable to this product.
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
interferon gamma
NCBI Official Synonym Full Names
interferon, gamma
NCBI Official Symbol
IFNG
NCBI Official Synonym Symbols
IFG; IFI
UniProt Protein Name
Interferon gamma
UniProt Synonym Protein Names
Immune interferon
Protein Family
Interferon
UniProt Gene Name
IFNG
UniProt Synonym Gene Names
IFN-gamma
UniProt Entry Name
IFNG_HUMAN
NCBI Protein Information
interferon gamma
NCBI Summary for IFNg
This gene encodes a member of the type II interferon family. The protein encoded is a soluble cytokine with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. Mutations in this gene are associated with aplastic anemia.[provided by RefSeq, Nov 2009]
UniProt Comments for IFNg
IFNG: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. Homodimer. Released primarily from activated T lymphocytes. Belongs to the type II (or gamma) interferon family.
Protein type: Membrane protein, integral; Cytokine; Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 12q14
Cellular Component: extracellular space; extracellular region; external side of plasma membrane
Molecular Function: interferon-gamma receptor binding; cytokine activity
Biological Process: positive regulation of isotype switching to IgG isotypes; positive regulation of nitric oxide biosynthetic process; negative regulation of smooth muscle cell proliferation; positive regulation of osteoclast differentiation; apoptosis; positive regulation of interleukin-23 production; positive regulation of interleukin-12 production; positive regulation of interleukin-6 biosynthetic process; negative regulation of epithelial cell differentiation; positive regulation of killing of cells of another organism; negative regulation of transcription from RNA polymerase II promoter; sensory perception of mechanical stimulus; positive regulation of interleukin-1 beta secretion; positive regulation of membrane protein ectodomain proteolysis; cell surface receptor linked signal transduction; positive regulation of MHC class II biosynthetic process; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; positive regulation of T cell proliferation; cell cycle arrest; defense response to virus; regulation of the force of heart contraction; positive regulation of peptidyl-serine phosphorylation of STAT protein; response to drug; neutrophil chemotaxis; positive regulation of synaptic transmission, cholinergic; adaptive immune response; CD8-positive, alpha-beta T cell differentiation during immune response; negative regulation of myelination; unfolded protein response; cytokine and chemokine mediated signaling pathway; response to virus; positive regulation of tumor necrosis factor production; defense response to protozoan; humoral immune response; antigen processing and presentation; positive regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of interleukin-12 biosynthetic process; negative regulation of interleukin-17 production; positive regulation of chemokine biosynthetic process; protein import into nucleus, translocation; defense response to bacterium; neutrophil apoptosis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of neuron differentiation; cell motility; regulation of insulin secretion
Disease: Hepatitis C Virus, Susceptibility To; Aplastic Anemia; Tuberous Sclerosis 2; Mycobacterium Tuberculosis, Susceptibility To; Human Immunodeficiency Virus Type 1, Susceptibility To
Show more Protein type: Membrane protein, integral; Cytokine; Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 12q14
Cellular Component: extracellular space; extracellular region; external side of plasma membrane
Molecular Function: interferon-gamma receptor binding; cytokine activity
Biological Process: positive regulation of isotype switching to IgG isotypes; positive regulation of nitric oxide biosynthetic process; negative regulation of smooth muscle cell proliferation; positive regulation of osteoclast differentiation; apoptosis; positive regulation of interleukin-23 production; positive regulation of interleukin-12 production; positive regulation of interleukin-6 biosynthetic process; negative regulation of epithelial cell differentiation; positive regulation of killing of cells of another organism; negative regulation of transcription from RNA polymerase II promoter; sensory perception of mechanical stimulus; positive regulation of interleukin-1 beta secretion; positive regulation of membrane protein ectodomain proteolysis; cell surface receptor linked signal transduction; positive regulation of MHC class II biosynthetic process; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; positive regulation of T cell proliferation; cell cycle arrest; defense response to virus; regulation of the force of heart contraction; positive regulation of peptidyl-serine phosphorylation of STAT protein; response to drug; neutrophil chemotaxis; positive regulation of synaptic transmission, cholinergic; adaptive immune response; CD8-positive, alpha-beta T cell differentiation during immune response; negative regulation of myelination; unfolded protein response; cytokine and chemokine mediated signaling pathway; response to virus; positive regulation of tumor necrosis factor production; defense response to protozoan; humoral immune response; antigen processing and presentation; positive regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of interleukin-12 biosynthetic process; negative regulation of interleukin-17 production; positive regulation of chemokine biosynthetic process; protein import into nucleus, translocation; defense response to bacterium; neutrophil apoptosis; positive regulation of transcription from RNA polymerase II promoter; positive regulation of neuron differentiation; cell motility; regulation of insulin secretion
Disease: Hepatitis C Virus, Susceptibility To; Aplastic Anemia; Tuberous Sclerosis 2; Mycobacterium Tuberculosis, Susceptibility To; Human Immunodeficiency Virus Type 1, Susceptibility To
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with IFNg elisa kit
| Reference Product | PubMed Publications |
|---|---|
| CD4 elisa kit | >153 publications with IFNg and CD4 |
| IL2 elisa kit | >58 publications with IFNg and IL2 |
| TBX21 elisa kit | >53 publications with IFNg and TBX21 |
| STAT1 elisa kit | >39 publications with IFNg and STAT1 |
| IFNGR1 elisa kit | >31 publications with IFNg and IFNGR1 |
| IL18 elisa kit | >23 publications with IFNg and IL18 |
| IRF1 elisa kit | >16 publications with IFNg and IRF1 |
| CD86 elisa kit | >6 publications with IFNg and CD86 |
Pathways associated with IFNg elisa kit
| Products by Pathway | Pathway Diagram |
|---|---|
| ATF-2 Transcription Factor Network Pathway antibodies | ATF-2 Transcription Factor Network Pathway Diagram |
| African Trypanosomiasis Pathway antibodies | African Trypanosomiasis Pathway Diagram |
| African Trypanosomiasis Pathway antibodies | African Trypanosomiasis Pathway Diagram |
| Allograft Rejection Pathway antibodies | Allograft Rejection Pathway Diagram |
| Allograft Rejection Pathway antibodies | Allograft Rejection Pathway Diagram |
| Allograft Rejection Pathway antibodies | Allograft Rejection Pathway Diagram |
| Amoebiasis Pathway antibodies | Amoebiasis Pathway Diagram |
| Amoebiasis Pathway antibodies | Amoebiasis Pathway Diagram |
| Antigen Processing And Presentation Pathway antibodies | Antigen Processing And Presentation Pathway Diagram |
| Antigen Processing And Presentation Pathway antibodies | Antigen Processing And Presentation Pathway Diagram |
Diseases associated with IFNg elisa kit
| Disease Name | Pubmed Publications |
|---|---|
| Necrosis Antibodies | >150 publications with IFNg and Necrosis |
| Autoimmune Diseases Antibodies | >87 publications with IFNg and Autoimmune Diseases |
| Respiratory Tract Diseases Antibodies | >70 publications with IFNg and Respiratory Tract Diseases |
| Hepatitis Antibodies | >33 publications with IFNg and Hepatitis |
| Lymphoproliferative Disorders Antibodies | >21 publications with IFNg and Lymphoproliferative Disorders |
| Diabetes Mellitus, Type 1 Antibodies | >17 publications with IFNg and Diabetes Mellitus, Type 1 |
| HIV Infections Antibodies | >17 publications with IFNg and HIV Infections |
| Hepatitis C Antibodies | >15 publications with IFNg and Hepatitis C |
| Arthritis, Rheumatoid Antibodies | >15 publications with IFNg and Arthritis, Rheumatoid |
| Leishmaniasis Antibodies | >11 publications with IFNg and Leishmaniasis |
Organs/Tissues associated with IFNg elisa kit
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Blood Antibodies | >483 publications with IFNg and Blood |
| Kidney Antibodies | >42 publications with IFNg and Kidney |
| Lymph Node Antibodies | >26 publications with IFNg and Lymph Node |
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