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> Chicken Immunoglobulin A,IgA ELISA Kit

Chicken Immunoglobulin A,IgA ELISA Kit

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Referência CSB-E11232Ch-24T

Tamanho : 24T

Marca : Cusabio

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Telefone : +1 850 650 7790

CSB-E11232Ch
Size 96T,5×96T,10×96T

Product Details

Target Name
Immunoglobulin A,IgA
Abbreviation
IgA
Species
Gallus gallus (Chicken)
Sample Types
serum, plasma
Detection Range
781.25 ng/mL-50000 ng/mL
Sensitivity
390 ng/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of chicken IgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
　 Sample Serum(n=4)  
1:100 Average % 97  
Range % 93-105  
1:200 Average % 94  
Range % 91-98  
1:400 Average % 102  
Range % 98-106  
1:800 Average % 88  
Range % 82-96  
Recovery
The recovery of chicken IgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 92-102  
EDTA plasma (n=4) 102 98-108  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
50000 0.072 0.071 0.072    
25000 0.134 0.136 0.135    
12500 0.231 0.242 0.237    
6250 0.426 0.435 0.431    
3125 0.666 0.675 0.671    
1562.5 0.938 0.927 0.933    
781.25 1.248 1.147 1.198    
0 1.695 1.594 1.645    
Materials provided
  • A 96-well Assay plate --The 96-well plate has been pre-coated with chicken IgA.
  • Standard(10 x concentrate) (1 x 200 μl) --Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
  • HRP-conjugated IgA antibody(100 x concentrate) (1 x 60 μl) --Bind to the IgA, and HRP catalyzes the TMB to elicit a chromogenic reaction.
  • HRP-conjugate Diluent (1 x 10 ml) --Dilute the HRP-conjugated IgA antibody solution.
  • Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration.
  • Wash Buffer (25x concentrate) (1 x 20 ml) --Wash away unbound or free substances.
  • TMB Substrate (1x 10 ml) --Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • Stop Solution (1 x 10ml) --Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells)
  • An Instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm - 570 nm.
  • An incubator that can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
ELISA Data Analysis
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
The Chicken Immunoglobulin A (IgA) ELISA Kit can be your reliable solution for measuring immunoglobulin A in Gallus gallus (chicken) samples. This ELISA kit is designed to detect and quantify IgA protein in serum and plasma samples with a wide detection range of 781.25 ng/mL-50000 ng/mL and a sensitivity of 390 ng/mL. This kit utilizes a competitive measurement principle, making it a highly quantitative assay for researchers in the field of immunology. The assay time is quick and efficient, taking only 1-5 hours to complete, and requires a small sample volume of 50-100ul. The detection...

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■ Q&A
Q:

For the kit CSB-E11232Ch， a customer asks if they have been tested with tissue samples, specifically small intestine.
Would you have this information available to share?

A:
Thanks for your inquiry.
We suggest you do a pretest if you want to test small intestine.Pls let me know if you have any further questions. Thank you.