Chicken Heat Shock Protein 70,Hsp-70 ELISA Kit
Referência CSB-E11196Ch-96T
Tamanho : 96T
Marca : Cusabio
Contactar o distribuidor local :
Telefone : +1 850 650 7790
| Size | 96T,5×96T,10×96T |
Product Details
Target Name
Heat Shock Protein 70,Hsp-70
Associated names
Heat shock 70 kDa protein ELISA Kit; HSP70 ELISA Kit
Abbreviation
HSP70
Uniprot No.
Species
Gallus gallus (Chicken)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.25 ng/mL-50 ng/mL
Sensitivity
0.125 ng/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Competitive
Precision
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken Hsp-70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 83-97 | |||||
| 1:2 | Average % | 91 | ||||
| Range % | 85-98 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 84-99 | |||||
Recovery
| The recovery of chicken Hsp-70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 98 | 92-104 | ||||
| EDTA plasma (n=4) | 96 | 90-102 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||
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ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
This Chicken HSP70 ELISA Kit was designed for the quantitative measurement of Chicken HSP70 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 0.25 ng/mL-50 ng/mL and the sensitivity is 0.125 ng/mL.
Citations
- RA Saad, RA Hasan, HMH Qutob, HS Ali,Journal of Evolutionary Biochemistry and Physiology,2025
- H Pertiwi,Italian Journal of Animal Science,2024
- AK Srihartini,/,/
- H Zhang,Poultry Science,2022
- J Son,Antioxidants,2022
- M. AG. Mustafa, et al,Iraqi journal of agricultural sciences,2019
- Kumar.et al,Nanaji Deshmukh Veterinary Science University,2017
- Qu Y,.et al,Poultry Science,2016
Customer Reviews and Q&A
There are currently no reviews for this product.
Target Background
Function
(From Uniprot)
(From Uniprot)
Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones....
Gene References into Functions
- data indicate that HSP70 is part of the receptor complex of Avian infectious bronchitis virus (IBV) and might help to understand the mechanism of S-mediated cell entry of IBV
- variation in chicken HSP70 was abundant between and within species
- The effects of transport time on HSP70 expression, stress, and meat quality are reported.
- Mn had a dosage-dependent effect on HSP27, HSP40, HSP60, HSP70, and HSP90 mRNA expression in chicken spleen lymphocytes in vitro.
- Results of this study indicated a long-term enhancement of Hsp70 gene expression associated with improved thermotolerance acquisition in treated chicks without adversely affecting performance.
- It was concluded that dietary supplementation of Z. officinale and Z. zerumbet powder may induce HSP70 reaction in broiler chickens exposed to heat stress.
- Data suggest that the expression of HSP70 is heat inducible and tissue dependent and that heat induction may correlate with DNA methylation pattern in the HSP70 promoter.
- The effects of human interaction on HSP70 expression and stress hormone blood leves in female broiler chickens are reported.
- It appears that neonatal feed restriction can enhance resistance to Salmonella enteritidis colonization in heat-stressed broiler chicks, and the underlying mechanism could be associated with the lower expression of Hsp70.
- HSP 70 level increased both in vivo and in vitro trials and may be used as potential molecular and toxicological biomarker.
- Spinal cord overexpression of Hsp70 enhances motor neuron survival in vivo during the period of naturally occurring cell death.
- A study evaluating the relationship between tissue or allele and the expression of chicken hsp70 under normal growth conditions and during acute heat stress is reported.
- The interaction of Meq and Hsp70 is significant in Marek's disease virus oncogenesis.
- The results suggest that the improved tolerance to transport stress in chicks could be associated with better HSP 70 expression.
Protein Families
Heat shock protein 70 family
Database Links
KEGG:
STRING:
UniGene: