CheKine™ Micro Lactate Dehydrogenase (LDH) Assay Kit
Referência KTB1110-96
Tamanho : 96T/96S
Marca : Abbkine
Contactar o distribuidor local :
Telefone : +1 850 650 7790
Specification
| Product name | CheKine™ Micro Lactate Dehydrogenase (LDH) Assay Kit |
| Applications notes | CheKine™ Micro Lactate Dehydrogenase Assay Kit provides a simple and easy colorimetric assay for measuring Lactate Dehydrogenase in serum, plasma, cell culture supernatants, tissue/cell lysates culture medium, fermentation and other biological fluids. In this colorimetric assay, LDH reduces NAD to NADH, which then interacts with a probe to produce a color (λmax = 450 nm). The assay is quick and convenient. |
Product Properties
| Kit components | • Assay Buffer • Lactate • NAD • WST-8 • Enhancer • Lactate Dehydrogenase Standard (1KU/mL) |
| Features & Benefits | • Optimized procedure for measuring Lactate Dehydrogenase (LDH) in various samples containing serum, plasma, cell culture supernatants, tissue/cell lysates culture medium, plant tissues,fermentation and other biological fluids. • Simple and easy colorimetric assay. • Quick and convenient. |
| Calibration range | 1- 20 U/mL |
| Usage notes | • Briefly centrifuge small vials at low speed prior to opening. • Performing several dilutions of your sample to ensure the readings are within the standard value range. • Use fresh samples. If not assayed immediately, samples can be stored at -80°C for one month. |
| Storage instructions | Storage at –20°C and Keep from light. Stable for at least 6 months at recommended temperature from date of shipment. |
| Shipping | Gel pack with blue ice. |
Additional Information
| Background | Lactate dehydrogenase (LDH) is an oxidoreductase (EC 1.1.1.27) presents in a wide variety of organisms. It catalyses the inter-conversion of pyruvate and lactate with concomitant inter-conversion of NADH and NAD+. It converts pyruvate, the final product of glycolysis, to lactate in hypoxic conditions. LDH quantification is of clinical interest as serum levels of certain LDH isozymes reflect pathological conditions in particular tissues. When disease or injury or toxic material damages tissues, the cells LDH is released into the bloodstream. Since LDH is a fairly stable enzyme, LDH has been widely used to evaluate the presence of damage and toxicity of tissue and cells. |
Image & description
-
View
Metabolic and immunomodulatory control of type 2 diabetes via generating cellular itaconate reservoirs by inflammatory-targeting gene-therapy nanovesicles(KTB1110-14.9)
Author：A Lao, W Li, Y Sun, Y Cao, Y Zhuang, J Wu, D Li, ... Publication name：Nanoscale Res Lett IF：14.9
-
View
A high-throughput Gaussia luciferase reporter assay for screening potential gasdermin E activators against pancreatic cancer(KTB1110-14.6)
Author：Y Liu, X Zhang, P Zhang, T He, W Zhang, D Ma, ... Publication name：Fish Physiol Biochem IF：14.6
-
View
Binary pyroptosis-amplified self-assembling prodrug nanomedicine enhances immunogenicity and inhibits abdominal metastasis in ovarian cancer(KTB1110-14.6)
Author：方锋 Publication name：Frontiers in Psychiatry IF：14.6
-
View
Mitochondrial CISD1 Modulates Microglial Metabolic Reprogramming to Drive Stress Susceptibility in Mice(KTB1110-14.1)
Author：W Dong, D Liu, S Fu, J Zhang, X Chen, ... Publication name：Nature Communications IF：14.1
-
View
Tryptophan deficiency induced by indoleamine 2, 3‐dioxygenase 1 results in glucose transporter 1‐dependent promotion of aerobic glycolysis in pancreatic cancer.(KTB1110-11)
Author：Liang, Heng, et al. Publication name：MedComm IF：11
-
View
Apabetalone, a BET protein inhibitor, inhibits kidney damage in diabetes by preventing pyroptosis via modulating the P300/H3K27ac/PLK1 axis.(KTB1110-9)
Author：Wang, Min, et al. Publication name：Pharmacological Research IF：9
-
View
TAB182 regulates glycolytic metabolism by controlling LDHA transcription to impact tumor radiosensitivity.(KTB1110-8)
Author：Chen, Shi, et al. Publication name：Cell Death & Disease IF：8
-
View
The Bcr-Abl inhibitor DCC-2036 inhibits necroptosis and ameliorates osteoarthritis by targeting RIPK1 and RIPK3 kinases(KTB1110-7.5)
Author：L Piao, D Wu, C Rui, Y Yang, S Liu, J Liu, Z Jin, ... Publication name：Front. Pharmacol IF：7.5
-
View
Targeting HDAC6 attenuates nicotine-induced macrophage pyroptosis via NF-κB/NLRP3 pathway(KTB1110-5.7)
Author：S Xu, H Chen, H Ni, Q Dai Publication name：Front Oncol IF：5.7
-
View
Calcium-mediated mitochondrial fission and mitophagy drive glycolysis to facilitate arterivirus proliferation(KTB1110-4.9)
Author：Z Sun, Z Ma, W Cao, C Jiang, L Guo, K Liu, ... Publication name：Autophagy IF：4.9
-
View
Circadian disruption by simulated shift work aggravates periodontitis via orchestrating BMAL1 and GSDMD-mediated pyroptosis(KTB1110-4.9)
Author：Y Wang, R Li, Q Ye, D Fei, X Zhang, J Huang, ... Publication name：Journal of Ethnopharmacology IF：4.9
-
View
Spreading of P301S Aggregated Tau Investigated in Organotypic Mouse Brain Slice Cultures(KTB1110-4.8)
Author：DS Korde, C Humpel Publication name：EBioMedicine IF：4.8
-
View
A Comparative Study on Low and High Salinity Tolerance of Two Strains of Pinctada fucata(KTB1110-4)
Author：J Sun, M Chen, Z Fu, J Yang, S Zhou, G Yu, ... Publication name：Brain Research Bulletin IF：4
-
View
Canagliflozin ameliorates NLRP3 inflammasome-mediated inflammation through inhibiting NF-κB signaling and upregulating Bif-1(KTB1110-4)
Author：Y Niu, Y Zhang, W Zhang, J Lu, Y Chen, ... Publication name：Neuromolecular Med IF：4
-
View
A Novel Prognostic Signature Integrating Immune and Glycolytic Pathways for Enhanced Prognosis and Immunotherapy Prediction in Hepatocellular Carcinoma(KTB1110-3.4)
Author：Z Zhang, H Zhao, P Wang, X Geng, M Yin, ... Publication name：Cells IF：3.4
-
View
Physical Responses of Pinctada fucata to Salinity Stress(KTB1110-3)
Author：J Yang, J Yang, M Chen, Z Fu, J Sun, G Yu, ... Publication name：Neuroscience IF：3
-
View
Spermidine mitigates lipopolysaccharide-induced acute lung injury by enhancing autophagic flux(KTB1110-1.9)
Author：X Zhang, H Lu, X Xu Publication name：Biomed Pharmacother IF：1.9
Reviews
There are no reviews yet.