cDNA Synthesis Kit
Referência HP100041
Tamanho : 10reactions
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cDNA Synthesis Kit
SKU
HP100041
cDNA Synthesis kit for miRNA—10 reactions
Citations
Lactate is an epigenetic metabolite that drives survival in model systems of glioblastoma
Torrini, Nguyen, Shu et al
Mol Cell (2022) 82 (16), 3061-3076.e6 DOI: 10.1016/j.molcel.2022.06.030
Torrini, Nguyen, Shu et al
Mol Cell (2022) 82 (16), 3061-3076.e6 DOI: 10.1016/j.molcel.2022.06.030
Cited by 118
AI Summary: OriGene’s cDNA synthesis kit was used to reverse transcribe RNA into cDNA for gene expression analysis by qPCR in this study investigating lactate metabolism in glioblastoma.
Snippet: 500ng of total RNA was reverse transcribed using cDNA synthesis kit (Origene). Gene expression was evaluated by qPCR using SYBR green RT-PCR kit (Applied
L-Carnitine Mitigates Trazadone Induced Rat Cardiotoxicity Mediated via Modulation of Autophagy and Oxidative Stress
Khedr, El-Feky, Werida
Cardiovasc Toxicol (2022)
Khedr, El-Feky, Werida
Cardiovasc Toxicol (2022)
Cited by 7
AI Summary: OriGene's cDNA synthesis kit was used to generate complementary DNA from extracted mRNA, enabling the analysis of gene expression related to autophagy and cardiotoxicity in the study.
Snippet: Complementary DNA (cDNA) was synthesized from extracted mRNA using cDNA synthesis kit (Origene Technologies, USA). The cDNA was amplified using SensiMix
ABCC5 facilitates the acquired resistance of sorafenib through the inhibition of SLC7A11-induced ferroptosis in hepatocellular carcinoma
Huang, Chen, Lu et al
Neoplasia (2021) 23 (12), 1227-1239 DOI: 10.1016/j.neo.2021.11.002
Huang, Chen, Lu et al
Neoplasia (2021) 23 (12), 1227-1239 DOI: 10.1016/j.neo.2021.11.002
Cited by 150
AI Summary: OriGene’s Reverse Transcription System Kit (#11801-025) was used in this study to synthesize cDNA for quantitative RT-PCR analysis, enabling the assessment of gene expression changes related to sorafenib resistance in hepatocellular carcinoma cells.
Snippet: quantitative RT-PCR (qRT-PCR) were carried out according to the manufacturer's protocols. Briefly, first-strand cDNA synthesis was carried out by using a Reverse Transcription System Kit (#11801-025, OriGene Technologies, Rockville, MD, USA). cDNA from various cell samples was amplified with specific primers (Table S2). Statistical analysis Data were analyzed using SPSS version 20.0 software (SPS
The Hypertension Related Gene G-Protein Coupled Receptor Kinase 4 Contributes to Breast Cancer Proliferation
Yue, Tran, Wang et al
Breast Cancer (Auckl) (2021) 15, 11782234211015753 DOI: 10.1177/11782234211015753
Yue, Tran, Wang et al
Breast Cancer (Auckl) (2021) 15, 11782234211015753 DOI: 10.1177/11782234211015753
Cited by 15
AI Summary: OriGene products contributed to this study by providing the GRK4 cDNA (Cat# SC119498) for re-expression experiments and lentiviral shRNA constructs (Cat# TL313236V) for GRK4 knockdown in breast cancer cell lines, enabling the researchers to assess the functional role of GRK4 in breast cancer proliferation.
Microglia Regulate Cell Genesis in a Sex-dependent Manner in the Neonatal Hippocampus
Nelson, Peketi, Lenz
Neuroscience (2021) 453, 237-255 DOI: 10.1016/j.neuroscience.2020.10.009
Nelson, Peketi, Lenz
Neuroscience (2021) 453, 237-255 DOI: 10.1016/j.neuroscience.2020.10.009
Cited by 23
AI Summary: In this study, OriGene products, specifically the Il1b (Cat# RQ025866) and Tnf (Cat# RQ025548) qPCR primers, were used to measure gene expression changes in the hippocampus following microglia depletion, helping to reveal the role of microglia in regulating cell genesis in a sex-dependent manner.
MET Inhibition Elicits PGC1α-Dependent Metabolic Reprogramming in Glioblastoma
Zhang, Nguyen, Shang et al
Cancer Res (2020) 80 (1), 30-43 DOI: 10.1158/0008-5472.CAN-19-1389
Zhang, Nguyen, Shang et al
Cancer Res (2020) 80 (1), 30-43 DOI: 10.1158/0008-5472.CAN-19-1389
AI Summary: In this study, OriGene products—including c-MET shRNA (OriGene, #TG311513), c-MET CRISPR/Cas9 knockout plasmid (OriGene, #KN203012), and PGC1α shRNA (OriGene, #TG312157)—were used to genetically inhibit c-MET and PGC1α, enabling the authors to demonstrate that c-MET inhibition leads to PGC1α-dependent metabolic reprogramming in glioblastoma cells.
Showing 1-6 of 47 papers.
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