CD8a (Ly-2 Lyt-2, Ly-35, Ly-B, T8) (APC/Cy5.5)

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Referência 228173-100ug

Tamanho : 100ug

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Telefone : +1 850 650 7790

Marca : US Biological

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Contactar o distribuidor local :

Telefone : +1 850 650 7790

228173 CD8a (Ly-2 Lyt-2, Ly-35, Ly-B, T8) (APC/Cy5.5)

Clone Type

Monoclonal

Host

rat

Source

mouse

Swiss Prot

P01731

Isotype

IgG2a,k

Grade

Purified

Applications

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

4°C Do Not Freeze

In the mouse, CD8 exists in two forms–a CD8 heterodimer composed of an a chain (CD8a) and a b chain (CD8b); and (ii) a homodimer of two a chains.The heterodimer is found on the surface of essentially all thymocytes and the “suppressor/cytotoxic” subpopulation of mature T lymphocytes. Subsets of intestinal intraepithelial lymphocytes express CD8a without CD8b. It has been suggested that CD8+b T cells mature extrathymically, while development of the CD8a+b+ population of T cells is thymus-dependent. CD8 acts as a coreceptor with MHC Class I- restricted T cell receptors in antigen recognition and positive selection of MHC class I-restricted CD8+ T cells.

In vivo and in vitro treatment with the 228173 antibody effectively depletes CD8α+ cells. 228173 also blocks allogeneic help specific for class I MHC antigens and T cell responses to IL-2.

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: ≤0.2µg/106 cells
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC/Cy5.5 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Applications

Product Type: Mab|Isotype: IgG2a|Clone No: 53-6.7|Host: rat|Source: mouse|Concentration: ~0.1mg/ml |Form: Supplied as a liquid in PBS, sodium azide. Labeled with Allophycocyanin (APC/Cy5.5). |Purity: Purified |Immunogen: Spleen cells or thymocyte membranes.|Specificity: Recognizes mouse CD8a.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Spleen cells or thymocyte membranes.

Form

Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizer. Labeled with Allophycocyanin (APC/Cy5.5).

Purity

Purified

Specificity

Recognizes mouse CD8a.

References

1. Ledbetter JA, Herzenberg LA. Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev. 1979;47:63-90. (Immunogen, IP, FC) 2.Konno A, Takada K, Saegusa J, Takiguchi M. Presence of B7-2+ dendritic cells and expression of Th1 cytokines in the early development of sialodacryoadenitis in the IQI/Jic mouse model of primary Sjörgren's syndrome. Autoimmunity. 2003;36:247-54. (IHC-FS) 3.Delong P, Tanaka T, Kruklitis R, Henry AC, Kapoor V, Kaiser LR, et al. Use of cyclooxygenase-2 inhibition to enhance the efficacy of immunotherapy. Cancer Res. 2003;63:7845-52. (IHC-FS) 4.Wehling-Henricks M, Jordan MC, Gotoh T, Grody WW, Roos KP, Tidball JG. Arginine metabolism by macrophages promotes cardiac and muscle fibrosis in mdx muscular dystrophy. PloS One. 2010;5 (5):e10763. (IHC-FS) 5.Kunikata N, Sano K, Honda M, Ishii K, Matsunaga J, Okuyama R, et al. Peritumoral CpG oligodeoxynucleotide treatment inhibits tumor growth and metastasis of B16F10 melanoma cells. J Invest Dermatol. 2004;123:395-402. (IHC-FS) 6.Ledbetter JA, Seaman WE, Tsu TT, Herzenberg LA. Lyt-2 and lyt-3 antigens are on two different polypeptide subunits linked by disulfide bonds. Relationship of subunits to T cell cytolytic activity. J Exp Med. 1981; 153:1503-16. (IP, FC, Block) 7.Choi J, Oh S, Lee D, Oh HJ, Park JY, Lee SB, et al. Mst1-FoxO signaling protects naïve T lymphocytes from cellular oxidative stress in mice. PloS One. 2009;4(11):e8011. (FC, ICC) 8.Hathcock KS. T cell depletion by cytotoxic elimination. Curr Protoc Immunol. 1991;3:4.1. (Depletion) 9.Lagrota-Candido J, Vasconcellos R, Cavalcanti M, Bozza M, Savino W, Quirico-Santos T. Resolution of skeletal muscle inflammation in mdx|dystrophic mouse is accompanied by increased immunoglobulin and interferon-γ production. Int J Exp Path. 2002;83:121-32. (FC 10.Parmo-Cabañas M, García-Bernal D, García-Verdugo R, Kremer L, Márquez G, Teixidó J. Intracellular signaling required for CCL25-stimulated T cell adhesion mediated by the integrin α4β1. J Leukoc Biol. 2007;82:380-91. (FC) 11. Fink LN, Frøkiær H. Dendritic cells from Peyer's patches and mesenteric lymph nodes differ from spleen dendritic cells in their response to commensal gut bacteria. Scand J Immunol. 2008;68:270-9. (FC) 12.Jordan KR, Buhrman JD, Sprague J, Moore BL, Gao D, Kappler JW, et al. TCR hypervariable regions expressed by T cells that respond to effective|tumor vaccines. Cancer Immunol Immunother. 2012;61:1627-38. (FC) 13. Elgbratt K, Jansson A, Hultgren-Hörnquist E. A quantitative study of the mechanisms behind thymic atrophy in Gαi2-deficient mice during colitis development. PLoS One. 2012;7(5):e36726. (FC) 14.Domingos-Pereira S, Decrausaz L, Derré L, Bobst M, Romero P, Schiller JT, et al. Intravaginal TLR agonists increase local vaccine-specific CD8 T cells and human papillomavirus-associated genital-tumor regression in mice. Mucosal Immunol. 2013;6:393-404. (FC) 15.Grodeland G, Mjaaland S, Roux KH, Fredriksen AB, Bogen B. DNA vaccine that targets hemagglutinin to MHC class II molecules rapidly induces antibody-mediated protection against influenza. J Immunol. 2013;191:3221-31. (FC)