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> CD73 Protein, Human, Recombinant (His & Avi), Biotinylated

CD73 Protein, Human, Recombinant (His & Avi), Biotinylated

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Referência NB-64-61692-20ug

Tamanho : 20ug

Marca : Neo Biotech

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CD73 Protein, Human, Recombinant (His & Avi), Biotinylated

(Synonyms: NTE, NT5, NT, eNT, eN, E5NT, CD73, CALJA, 5'-nucleotidase, ecto (CD73)) Copy Product Info

Synonyms: NTE, NT5, NT, eNT, eN, E5NT, CD73, CALJA, 5'-nucleotidase, ecto (CD73)

Catalog No. TMPY-06907 Copy Product Info
CD73 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 61.04 kDa and the accession number is NP_002517.1.
Other Forms of CD73 Protein, Human, Recombinant (His & Avi), Biotinylated:
NTS1 Protein, Mouse, Recombinant (hFc)
Neurotensin Protein, Human, Recombinant (hFc)
CD73/5'-Nucleotidase Protein, Mouse, Recombinant (His)
CD73/5'-Nucleotidase Protein, Human, Recombinant (His)
CD73/5'-Nucleotidase Protein, Cynomolgus, Recombinant (His)
CD73/5'-Nucleotidase Protein, Rat, Recombinant (His)
CD73/5'-Nucleotidase Protein, Human, Recombinant (hFc)
NT-4 Protein, Human, Recombinant
For research use only—not for human use. No sales to individuals. Use as intended only.
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Purity:SDS-PAGE: 96.5%; SEC-HPLC: 92.5%
Appearance:Lyophilized powder
Color:White
Contact us for more batch information

Product Introduction

Bioactivity
Bioactivity
Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
CD73 Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 61.04 kDa and the accession number is NP_002517.1.
Species
Human
Expression System
HEK293 Cells
TagC-His-Avi
Accession NumberNP_002517.1
ConstructionA DNA sequence encoding the human NT5E (NP_002517.1) (Met1-Lys547) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. Predicted N terminal: Trp 27
Protein Purity
≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 120 mM Nacl, 4 mM Cacl2, 20% Glycerol, pH 7.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionReconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
SynonymsNTE, NT5, NT, eNT, eN, E5NT, CD73, CALJA, 5'-nucleotidase, ecto (CD73)
Research Background
5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein that belongs to the 5'-nucleotidase family. CD73 is a glycosylphosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signaling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1, and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purinergic receptors to induce cellular responses. CD73 has also been reported to regulate the expression of pro-inflammatory molecules in mouse endothelium.
Chemical Properties
Molecular Weight61.04 kDa (predicted); 60.6 kDa (reducing conditions)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

NT-5NT 5CD-73CD 735'-nucleotidase, ecto
Related Tags: CD73 Protein, Human, Recombinant (His & Avi), Biotinylated chemical structure | CD73 Protein, Human, Recombinant (His & Avi), Biotinylated molecular weight