CD133/PROM1 Protein, Rat, Recombinant (His)

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Referência NB-64-58761-50ug

Tamanho : 50ug

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CD133/PROM1 Protein, Rat, Recombinant (His)

(Synonyms: prominin 1) Copy Product Info

Synonyms: prominin 1

Catalog No. TMPY-03691 Copy Product Info

CD133/PROM1 Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 37.10 kDa and the accession number is Q7TSL4.

For research use only—not for human use. No sales to individuals. Use as intended only.

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Purity:> 90 % as determined by SDS-PAGE

Appearance:Lyophilized powder

Color:White

Product Introduction

Bioactivity

Bioactivity	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	CD133/PROM1 Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 37.10 kDa and the accession number is Q7TSL4.
Species	Rat
Expression System	HEK293 Cells
Tag	N-His
Accession Number	Q7TSL4
Construction	A DNA sequence encoding the rat PROM1 (NP_001103607.1) (Asn171-Tyr424) was expressed with a polyhistidine tag at the N-terminus. Predicted N terminal: His
Protein Purity	> 90 % as determined by SDS-PAGE
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
Synonyms	prominin 1
Research Background	CD133, also known as PROM1 and Prominin 1, is a pentaspan transmembrane glycoprotein that belongs to the prominin family. It localizes to membrane protrusions and is often expressed on adult stem cells. CD133 is known to play a role in maintaining stem cell properties by suppressing differentiation. CD133 binds cholesterol in cholesterol-containing plasma membrane microdomains. It is proposed to play a role in apical plasma membrane organization of epithelial cells. CD133 is also involved in regulation of MAPK and Akt signaling pathways. Mutations in PROM1 gene have been shown to result in retinitis pigmentosa and Stargardt disease. PROM1 gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Expression of this gene is also associated with several types of cancer.

Chemical Properties

Molecular Weight	37.10 kDa (predicted); 47-57 kDa (reducing conditions)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Related Tags: CD133/PROM1 Protein, Rat, Recombinant (His) chemical structure | CD133/PROM1 Protein, Rat, Recombinant (His) molecular weight