Quimigen Portugal > CD11a (ITGAL) mouse monoclonal antibody, clone MEM-83, Azide Free
CD11a (ITGAL) mouse monoclonal antibody, clone MEM-83, Azide Free
Referência SM3100AS
Tamanho : 100ug
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CD11a (ITGAL) Mouse Monoclonal Antibody [Clone ID: MEM-83]
| Product Data | |
| Clone Name | MEM-83 |
|---|---|
| Application | FC, FN, IP |
| Recommended Dilution | Functional Application: The antibody MEM-83 directly induces the binding of T cells to purified ICAM-1. Using an in vitro-translated CDlla cDNA deletion series, the MEM-83 activation epitope was mapped to the "I" domain of the LFA-1 a subunit. The studies have therefore identified a novel LFA-1 activation epitope mapping to the I domain of LFA-1, which could play a role in the regulation of LFA-1 binding to ICAM-1. Flow Cytometry: 1 µg/ml. Immunoprecipitation. |
| Reactivity | Human |
| Antibody Host | Mouse |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Immunogen | Human peripheral blood lymphocytes. |
| Specificity | The antibody MEM-83 reacts with CD11a (alpha subunit of Human LFA-1), a 170-180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils. |
| Buffer | Azide Free PBS, pH~7.4 preserved by filter sterilization (0.2 µM filtration) State: Azide Free State: Liquid purified Ig fraction (> 95 pure by SDS-PAGE) Preservative: None |
| Concentration | lot specific |
| Purification | Affinity Chromatography on Protein A |
| Conjugation | Unconjugated |
| Storage | Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. |
| Stability | Shelf life: one year from despatch. |
| Shipping | Blue Ice |
| Gene Name | integrin subunit alpha L |
| Database Link | |
| Background | CD11a (LFA-1a) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the aLb2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation; binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity. |
| Synonyms | Integrin alpha-L, LFA1, LFA-1 |
| Reference Data | |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.