Tamanho : 25mg
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Synonyms: N-hexanoyl-NBD-Galactosylceramide, NBD GalCer(d18:1/6:0), NBD Galactosylceramide (d18:1/6:0), C6-NBD-GalCer
| Description | C6 NBD galactosylceramide, an active derivative of galactosylceramide tagged with fluorescent nitrobenzoxadiazole (NBD), serves as a substrate for neutral β-glycosylceramidase (GCase) in studies of intracellular localization and metabolism of galactosylceramide (Ex=nm, Em=525) [1]. |
| In vitro | C6-NBD-glucosylceramide (4 μM) undergoes transport to the Golgi apparatus in HT29 cells [1]. According to our recommended protocol, transcytosis of exogenous C6-NBD-GaICer follows these steps after endocytosis: Initially, C6-NBD-glucosylceramide is inserted at 10°C. Subsequently, cells are subjected to three cold HBSS rinses and maintained at 37°C in HBSS to facilitate endocytosis. After 10 minutes, residual probe on the cell surface is eliminated through either two (apical) or three (basal) 20-minute BSA washes at 10°C. Lipid analysis using one set of filters quantifies the endocytosis, while a second set incubated for either 0.5 or 1 hour at 37°C in HBSS + BSA measures the reemergence of intracellular C6-NBD-glucosylceramide on the cell surfaces. Post-incubation, a 10°C BSA wash is conducted before extracting the NBD lipids into chloroform/methanol from both apical and basal media, as well as the cells, which are then analyzed and quantitated by TLC. The above information is based on published literature. Experimental procedures should be appropriately modified to meet specific research demands. |
| Synonyms | N-hexanoyl-NBD-Galactosylceramide, NBD GalCer(d18:1/6:0), NBD Galactosylceramide (d18:1/6:0), C6-NBD-GalCer |
| Molecular Weight | 737.88 |
| Formula | C36H59N5O11 |
| Cas No. | 170212-26-7 |
| Smiles | N(CCCCCC(N[C@@H](CO[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O)[C@@H](/C=C/CCCCCCCCCCCCC)O)=O)C=2C=3C(C(N(=O)=O)=CC2)=NON3 |
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. |
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 µL Tween 80 and mix well until fully clarified.
3) Add 450 µL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
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