C5 ELISA Kit (Monkey)
Referência OKCA02322
Tamanho : 96Wells
Marca : Aviva Systems Biology
Contactar o distribuidor local :
Telefone : +1 850 650 7790
C5 ELISA Kit (Monkey) (OKCA02322)
| Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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| Predicted Species Reactivity | Monkey | ||||||||||||||||||||||
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| ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
| ELISA Kit Duration | ~ 3.5 Hours | ||||||||||||||||||||||
| ELISA Kit Principle | Aviva Systems Biology C5 ELISA Kit (Monkey) (OKCA02322) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for C5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for C5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard C5 captured in the well. | ||||||||||||||||||||||
| ELISA Kit Range | 0.625 - 40 ng/mL | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Mean Intra-Assay: CV <12% (n=20) Mean Inter-Assay: CV <15% (n=24) | ||||||||||||||||||||||
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| Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
| Sample Type | Serum, Plasma, Tissue Homogenates | ||||||||||||||||||||||
| Sensitivity | 0.156 ng/mL | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich Immunoassay |
| Gene Symbol | C5 |
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| Alias Symbols | CPAMD4, FLJ17816, FLJ17822, MGC142298, anaphylatoxin C5a analog |
| Protein Name | Complement C5a anaphylatoxin |
| Description of Target | Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. Binding to the receptor C5AR1 induces a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation. |
| Uniprot ID | P12082 |
