Principle of the Assay: The principle of the following enzyme immunoassay test follows a typical two-step capture or 'sandwich' type assay. The assay makes use of two highly specific monoclonal antibodies: A monoclonal antibody specific for CRP is immobilized onto the microwell plate and another monoclonal antibody specific for a different region of CRP is conjugated to horse radish peroxidase (HRP). CRP from the sample and calibrators are allowed to bind to the plate, washed, and subsequently incubated with the HRP conjugate. After a second washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed by the enzymatic reaction is directly proportional to the concentration of CRP in the sample. A set of calibrators is used to plot a standard curve from which the amount of CRP in samples and controls can be directly read.

Intended Uses: For the determination of C-Reactive Protein by enzyme immunoassay in human serum.

Small volumes of hsCRP elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS494066 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the C-Reactive Protein (hsCRP) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing hsCRP. The ELISA analytical biochemical technique of the MBS494066 kit is based on hsCRP antibody-hsCRP antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect hsCRP antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, hsCRP. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). C-Reactive Protein (hsCRP) ELISA kit

C reactive protein (CRP) is a ring-shaped, protein and a member of the pentraxin family of proteins. It is synthesized in the liver in response to factors released by macrophages and fat cells (adipocytes)
Levels of plasma CRP rise in response to inflammation (e.g. following interleukin-6 secretion from macrophages and T cells). CRP binds to lysophosphatidylcholine that is expressed on the surface of dead or dying cells in order to activate the complement system via the C1Q complex. This response is referred to as the acute phase response initiated by a rise in the concentration of IL-6, originating from macrophages and/ or adipocytes. This acute phase response occurs in reaction to a wide range of acute and chronic inflammatory conditions such as bacterial, viral, or fungal infections; rheumatic and other inflammatory diseases; malignancy; and tissue injury and necrosis. These conditions cause release of interleukin-6 and other cytokines that trigger the synthesis of CRP and fibrinogen by the liver..

The detection and concentration of CRP in the a test sample e.g. plasma, serum can be derived from a standard curve. The details of reagents in this ELISA kit can be found in the product datasheet. Applications include both western blotting, antibody arrays and ELISA development for research purposes such target identification.