Bovine Immunoglobulin M,IgM ELISA Kit
Referência CSB-E12017B-96T
Tamanho : 96T
Marca : Cusabio
Contactar o distribuidor local :
Telefone : +1 850 650 7790
| Size | 96T,5×96T,10×96T |
Product Details
Target Name
Immunoglobulin M
Abbreviation
IgM
Species
Bos taurus (Bovine)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
0.39 μg/mL-25 μg/mL
Sensitivity
0.2 μg/mL
Assay Time
1-5h
Sample Loading Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Competitive
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 91 | ||||
| Range % | 86-99 | |||||
| 1:200 | Average % | 97 | ||||
| Range % | 92-103 | |||||
| 1:400 | Average % | 85 | ||||
| Range % | 81-87 | |||||
| 1:800 | Average % | 89 | ||||
| Range % | 83-95 | |||||
Recovery
| The recovery of bovine IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-97 | ||||
| EDTA plasma (n=4) | 97 | 92-102 | ||||
Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
ELISA Data Analysis
Troubleshooting
and FAQs
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Shelf Life
6 months
Description
Immunoglobulin M (IgM) is the largest antibody isotype and the first immunoglobulin produced during immune responses. This pentameric molecule serves as the primary antibody in acute infections. It plays an important role in complement activation and agglutination reactions. IgM levels are important indicators of immune system function and are frequently measured in clinical and research settings to assess immune status, monitor disease progression, and evaluate vaccine responses in various species including cattle. The Bovine Immunoglobulin M ELISA Kit (CSB-E12017B) provides quantitative...
Citations
- MSA Khattab, L Haiqiang, T Shaoxun,Frontiers in Veterinary Science,2025
- Y Zhu, Z Li, Y Yang, T Zhan, D Bu, L Ma,Journal of Agriculture and Food Research,2024
- K Kara,Tropical animal health and production,2023
- F Ma,Animals,2024
- A Wernicki,Acta Veterinaria Brno,2023
- Tropical study on Clinical and Host Cell Responses of Acute Phase Proteins, Pro-Inflammatory Cytokines and Antibodies’ in Lactating Dairy Cows Vaccinated and Challenged with S. aureus IU Hambali,ResearchGate,2022
- M Nguse,Biology,2022
Customer Reviews and Q&A
There are currently no reviews for this product.