Blood Ammonia Colorimetric Assay Kit
Referência E-BC-K145-S-100
Tamanho : 100Assays
Marca : Elabscience
Contactar o distribuidor local :
Telefone : +1 850 650 7790
For research use only.
| Detection Principle | Blood protein can be precipitated with protein precipitator, and enzyme activity will be destroyed, which can prevent the formation of free ammonia in vitro. Most interfering color substances were removed at the same time, indigo was formed in non-protein filtrate by Berthelot reaction, and the color depth was proportional to the content of blood ammonia. Blood ammonia content can be determined by comparing with standard solution. |
| Sample Type | Serum,Plasma |
| Detection Method | Colorimetric method |
| Detection Instrument | Spectrophotometer (635 nm) |
| Research Area | Liver And Renal Function , Amino Acids And Proteins |
| Other Reagents Required | Normal saline (0.9% NaCl) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.01 mmol/L |
| Detection Range | 0.01-2.0 mmol/L |
| Precision | inter-assay CV: 5% | intra-assay CV: 4.7% |
| Sample Volume | 0.2 mL |
| Assay Time | 1 h 10 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 2-6 |
| Rat serum | 1 |
| Rabbit serum | 1 |
| Mouse serum | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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