Tamanho : 10mg
Synonyms:
| Description | Bleomycin hydrochloride is a glycopeptide antibiotic and a DNA synthesis inhibitor. Bleomycin Sulfate can cause DNA strand breaks but not RNA strand breaks. Bleomycin Sulfate has anti-tumor activity. |
| Targets & IC50 | UT-SCC-19A cells:4.0 ± 1.3 nM, UT-SCC-12B cells:13.0 ± 1.1 nM, UT-SCC-12A cells:14.2 ± 2.8 nM |
| In vitro | Method: Human pancreatic cells (PCCs) AsPC-1 and MIA PaCa-2 were treated with Bleomycin Sulfate (0.1-100 µM) for 24-72 hours, and the cell growth inhibition was detected by the MTT method. Result: AsPC-1 cells showed no response to Bleomycin Sulfate. Bleomycin Sulfate significantly inhibited the cell growth of MIA-PaCa-2, and the IC50 at 24/48/72 h was 5.9/6.4/2.6 μM, respectively. [1] Method : Mesenchymal stem cells (MSCs) were treated with Bleomycin Sulfate (1800 ng/mL) for 24-96 hours, and sub-G1 and caspase-3 were detected by Flow Cytometry. Result: Bleomycin Sulfate induces upregulation of sub-G1 and caspase-3 activation, indicating that Bleomycin Sulfate induces apoptosis. [2] Method : Normal rat alveolar macrophages were incubated with Bleomycin Sulfate (0.01-1 μg/mL) for 18 hours, and their levels were determined using the MDGF Assay. Result: Bleomycin Sulfate stimulates macrophages to produce fibroblast growth factor (MDGF). [3] |
| In vivo | Method: To detect the anti-tumor activity in vivo, Bleomycin Sulfate (20 mg/kg) was intraperitoneally injected into BALB/c mice carrying mouse colorectal cancer tumor CT26 shControl or shCRT, once every two days for five times. Result: Bleomycin Sulfate delayed the growth of CT26 cells transfected with control shRNA, but had no effect on CT26 cells transfected with CRT shRNA. [4] Method: To study the time course of Bleomycin induced pulmonary fibrosis in mice, Bleomycin Sulfate (0.06 mg/ mouse in 0.9% saline) was administered as a single intratracheal instillation (IT) to C57Bl/6J mice. Result: Bleomycin Sulfate induced pulmonary fibrosis in mice. The most appropriate time point for evaluating pulmonary fibrosis in this model is 14 days after IT infusion of Bleomycin Sulfate. This is based on the observation that extensive fibrosis occurred in animals at 14 days, but the variability of the fibrotic response was smaller and the mortality rate was lower than 21 days. [5] |
| Disease Modeling Protocol | Pulmonary fibrosis model
*Precautions： All mice were sacrificed 14 days after modeling (day 14). *References：Chen Y,et,al. Nicotinamide phosphoribosyltransferase prompts bleomycin-induced pulmonary fibrosis by driving macrophage M2 polarization in mice. Theranostics. 2024 Apr 28;14(7):2794-2815. |
| Molecular Weight | 1451.005 |
| Formula | C55H84ClN17O21S3 |
| Cas No. | 67763-87-5 |
| Smiles | [Cl-].C[C@@H](O)[C@H](NC(=O)[C@@H](C)[C@H](O)[C@@H](C)NC(=O)[C@@H](NC(=O)C1=C(C)C(N)=NC(=N1)[C@H](CC(N)=O)NC[C@H](N)C(N)=O)[C@@H](O[C@@H]1O[C@@H](CO)[C@@H](O)[C@H](O)[C@@H]1O[C@H]1O[C@H](CO)[C@@H](O)[C@H](OC(N)=O)[C@@H]1O)C1=CN=CN1)C(=O)NCCC1=NC(=CS1)C1=NC(=CS1)C(=O)NCCC[S+](C)C |
| Relative Density. | no data available |
| Storage | Store at low temperature,Keep away from direct sunlight,Keep away from moisture, Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. | ||||||||||||||||||||||||||||||||||||||||
| Solubility Information | DMSO: 255.00 mg/mL (175.74 mM), Sonication and heating to 60℃ are recommended.  H2O: 80.00 mg/mL (55.13 mM), Sonication is recommended. | ||||||||||||||||||||||||||||||||||||||||
| In Vivo Formulation | 10% DMSO+90% Saline: 10.00 mg/mL (6.89 mM), Solution. 10% DMSO+40% PEG300+5% Tween 80+45% Saline: 5.00 mg/mL (3.45 mM), Sonication is recommended. Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions. | ||||||||||||||||||||||||||||||||||||||||
Solution Preparation Table | |||||||||||||||||||||||||||||||||||||||||
H2O/DMSO
DMSO
Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density. | |||||||||||||||||||||||||||||||||||||||||
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 µL Tween 80 and mix well until fully clarified.
3) Add 450 µL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
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