Avidin-Biotin Blocking Kit
Referência E08-18
Tamanho : 18ml
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Avidin-Biotin Blocking Kit
SKU
E08-18
This product is designed to eliminate the non-specific binding caused by the endogenous avidin or biotin like substances in immunohistochemistry and cytochemistry, when either avidin or Streptavidin/biotin is used as the signal amplification system.
Citations
The AMPK-Sirtuin 1-YAP axis is regulated by fluid flow intensity and controls autophagy flux in kidney epithelial cells
Claude-Taupin, Isnard, Bagattin et al
Nat Commun (2023) 14 (1), 8056 DOI: 10.1038/s41467-023-43775-1
Claude-Taupin, Isnard, Bagattin et al
Nat Commun (2023) 14 (1), 8056 DOI: 10.1038/s41467-023-43775-1
Cited by 55
AI Summary: In this study, the OriGene Avidin/Biotin Blocking Kit (E08-18) was used to prepare tissue sections for YAP protein detection, thereby facilitating the investigation of YAP/TAZ's role in regulating autophagy in kidney epithelial cells under different fluid flow conditions.
Snippet: 200 dilution) or SQSTM1 (PROGEN, GP62-C, 1:100) antibody overnight at 4 °C, followed with the appropriate secondary antibody. For YAP detection, sections were first treated with avidin/biotin kit (Origene, E08-18) before using YAP primary antibody (Cell Signaling Technology, #14074, 1:200) overnight at 4 °C. Sections were then incubated with biotinylated anti-rabbit antibody (GEHealthcare, R
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