Anti-Zebrafish Beta Actin/ACTB Antibody Picoband® HRP Conjugated
Referência AZQ7ZVI7-HRP
Tamanho : 100ug/vial
Marca : Boster Bio
Contactar o distribuidor local :
Telefone : +1 850 650 7790
Tested Applications
ACTB antibody was positively detected by WB in:
zebrafish head tissue, female zebrafish viscera tissue, male zebrafish viscera tissue, whole zebrafish tissue, zebrafish embryo tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ACTB antibody was positively detected by IHC in:
zebrafish skeletal muscle tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Zebrafish
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Zebrafish
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Zebrafish Beta Actin/ACTB Antibody Picoband®
View all ACTB Antibodies
View all ACTB Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of zebrafish Beta Actin/ACTB.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
42 kDa
Background of ACTB
The primary site of action of cytochalasin B on cell motility processes is beta-actin. Habets et al.(1992) generated hybrids that harbor only specific regions of human chromosome 7 and assigned the ACTB locus to 7p15-p12. ACTB and the other assigned beta-actin-related sequences are dispersed over at least four different chromosomes including one locus assigned to the X chromosome. A mutation of beta-actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia.
Cite This Product
Anti-Zebrafish Beta Actin/ACTB Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZQ7ZVI7)
Protocols
Western Blot protocol for ACTB antibody
Immunohistochemistry (IHC) protocol for ACTB antibody
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