Anti-UGCG Antibody

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Referência CPA2209-30ul

Tamanho : 30ul

Contactar o distribuidor local :

Telefone : +1 850 650 7790

Marca : Cohesion Biosciences

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Contactar o distribuidor local :

Telefone : +1 850 650 7790

Western blot analysis of UGCG expression in mouse spleen (A), SHSY5Y (B) whole cell lysates. (Predicted band size: 44 kD; Observed band size: 60 kD)
Immunofluorescent analysis of UGCG staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).

Product Name:	Anti-UGCG Antibody
Cat No:	CPA2209
Source:	Rabbit
Reactivity:	H, M, R, B, P
Applications:	WB, IF/IC
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference

Description：Rabbit polyclonal antibody to UGCG

Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human UGCG. The exact sequence is proprietary.

Purification：The antibody was purified by immunogen affinity chromatography.

Specificity：Recognizes endogenous levels of UGCG protein.

Clonality：Polyclonal

Form：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution：WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)

Gene Symbol：UGCG

Alternative Names：Ceramide glucosyltransferase; GLCT-1; Glucosylceramide synthase; GCS; UDP-glucose ceramide glucosyltransferase; UDP-glucose:N-acylsphingosine D-glucosyltransferase

Entrez Gene (Human)： 7357;

Entrez Gene (Mouse)： 22234;

Entrez Gene (Rat)： 83626;

SwissProt (Human)： Q16739;

SwissProt (Mouse)： O88693;

SwissProt (Rat)： Q9R0E0;

Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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