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> Anti-Tryptophan Hydroxylase 1 Antibody (7R847)

Anti-Tryptophan Hydroxylase 1 Antibody (7R847)

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Referência NB-64-123986-100uL

Tamanho : 100uL

Marca : Neo Biotech

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Anti-Tryptophan Hydroxylase 1 Antibody (7R847)

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Catalog No. TMAY-02149 Copy Product Info
Anti-Tryptophan Hydroxylase 1 Antibody (7R847) is a Rabbit antibody targeting Tryptophan Hydroxylase 1. Anti-Tryptophan Hydroxylase 1 Antibody (7R847) can be used in IP,WB.
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Information

Bioactivity
Description
Anti-Tryptophan Hydroxylase 1 Antibody (7R847) is a Rabbit antibody targeting Tryptophan Hydroxylase 1. Anti-Tryptophan Hydroxylase 1 Antibody (7R847) can be used in IP,WB.
Ig Type
Rabbit IgG
Clone
7R847
Reactivity
Human
Specificity
Human Tryptophan Hydroxylase 1/TPH1
Verified Activity
1. TPH1 was immunoprecipitated using:
-Lane A:0.5 mg Jurkat Whole Cell Lysate.
-Lane B:0.5 mg THP-1 Whole Cell Lysate
-0.5 µL anti-TPH1 rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein G.
-Primary antibody:
-Anti-TPH1 rabbit monoclonal antibody, at 1:500 dilution.
-Secondary antibody:
-Clean-Blotô IP Detection Reagent (HRP) at 1:500 dilution.
-Developed using the DAB staining technique.
-Performed under reducing conditions.
-Predicted band size: 55 kDa.
-Observed band size: 60 kDa.
2. Anti-TPH1 rabbit monoclonal antibody at 1:500 dilution.
-Lane A: THP1 Whole Cell lysate.
-Lysates/proteins at 30 μg per lane.
-Secondary
-Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.
-Developed using the Odyssey technique.
-Performed under reducing conditions.
-Predicted band size:55 kDa.
-Observed band size:55 kDa(We are unsure as to the identity of these extra bands.)
Application
IP
WB
Recommended Dose
WB: 1:500-1:1000; IP: 0.2-1 μL/mg of lysate
Antibody Type
Monoclonal
Host SpeciesRabbit
ConstructionThis antibody was obtained from a rabbit immunized with purified, recombinant Human Tryptophan Hydroxylase 1 / TPH1 (rh Tryptophan Hydroxylase 1 / TPH1; TMPY-01959; P17752-1; Ile2-Ile444).
PurificationProtein A
AppearanceLiquid
Formulation0.2 μm filtered solution in PBS
Research BackgroundTryptophan 5-hydroxylase 1, also known as Tryptophan 5-monooxygenase 1, Tryptophan hydroxylase 1, TPH1, TPH and TPRH, is an enzyme that belongs to the biopterin-dependent aromatic amino acid hydroxylase family. TPH1 contains one ACT domain. Tryptophan hydroxylase catalyzes the biopterin-dependent monooxygenation of tryptophan to 5-hydroxytryptophan (5HT), which is subsequently decarboxylated to form the neurotransmitter serotonin. It is the rate-limiting enzyme in the biosynthesis of serotonin. It is the rate-limiting enzyme in the biosynthesis of serotonin. TPH1 expression is limited to a few specialized tissues: raphe neurons, pinealocytes, mast cells, mononuclear leukocytes, beta-cells of the islets of Langerhans, and intestinal and pancreatic enterochromaffin cells. The tryptophan hydroxylase 1 (TPH1) gene is also reported to be associated with suicidal behavior. Polymorphisms of TPH1 may assist in identifying a subgroup of mood disorder patients that is at higher risk for suicidal behavior.
Related Conjugates and Formulations
Conjucates
Unconjugated
Antigen Details
Immunogen
Recombinant Protein: Human Tryptophan Hydroxylase 1 / TPH1 Protein (TMPY-01959)
Antigen Species
Human
Biology Area
Neurotransmitter Associated Enzymes
Stability & Storage
Stability & StorageStore at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free.
TransportShipping with blue ice.

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Keywords

Tryptophan 5-monooxygenase 1Tryptophan 5-hydroxylase 1Tph1Tph 1Tph
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