Anti-SARS-CoV-2 Nucleocapsid (N) (Clone NP1-E9) - HRP

SARSCoV2 Nucleocapsid (N) Protein

This HRPconjugated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 7.4, 1% BSA. (Warning: Use of sodium azide as a preservative will inhibit the enzyme activity of horseradish peroxidase)

This horseradish peroxidase conjugated monoclonal antibody is stable when stored at 28°C. Do not freeze.

Ships Overnight on Blue Ice

AntiSARSCoV2 Nucleocapsid, clone NP1E9, specifically targets an epitope on the SARSCoV2 nucleocapsid protein. Futhermore, it is reported to bind to the oligomerization domain of the N protein.

Severe acute respiratory syndrome coronavirus 2 (SARSCoV2), the causative agent of coronavirus disease 2019 (COVID19), is an enveloped, singlestranded, positivesense RNA virus that belongs to the Coronaviridae family ¹. The SARSCoV2 genome, which shares 79.6% identity with SARSCoV, encodes four essential structural proteins: the spike (S), envelope (E), membrane (M), and nucleocapsid protein (N) ². The N protein is 46 kDa and consists of two highly conserved structural domains, the Nterminal domain (NTD) and Cterminal domain (CTD), connected by a linker region. The NTD and CTD are involved in RNA binding and selfoligomerization, respectively ^{3, 4}. The primary function of the N protein is to bind to and package the viral RNA genome into a helical ribonucleoprotein complex ⁵. The N protein is also involved in other critical steps of the viral life cycle, including transcription, replication, and modulating infected cell signaling pathways ^{6, 7}. The N protein is abundantly expressed during infection and is highly conserved, sharing 90% amino acid homology with the SARSCoV N protein ⁸. It is also immunogenic, and antibodies ^8,9 and memory T cells ^{10, 11} targeting the N protein are present in the sera of convalescent COVID19 patients, identifying the N protein as a suitable candidate for vaccine development and diagnostic assays. Diagnostic assays based on the N protein effectively detect antibodies in the sera of patients infected with SARSCoV2 ¹². The N protein also contributes to immune evasion by antagonizing antiviral RNAi ¹³, suggesting its potential value as a targeted therapeutic.

The nucleocapsid protein is expressed in the internal nucleocapsid of SARSCoV2.

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