Anti-PPAR gamma Antibody

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Referência CPA4778-200ul

Tamanho : 200ul

Contactar o distribuidor local :

Telefone : +1 850 650 7790

Marca : Cohesion Biosciences

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Contactar o distribuidor local :

Telefone : +1 850 650 7790

Western blot analysis of PPAR gamma expression in Hela (A), rat ovary (B), mouse spleen (C), LO2 (D) whole cell lysates. (Predicted band size: 57 kD; Observed band size: 57 kD)
Immunohistochemical analysis of PPAR gamma staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of PPAR gamma staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Product Name:	Anti-PPAR gamma Antibody
Cat No:	CPA4778
Source:	Rabbit
Reactivity:	H, M, R, D, Rb
Applications:	WB, IH, IF/IC
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference

Description：Rabbit polyclonal antibody to PPAR gamma

Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PPAR gamma. The exact sequence is proprietary.

Purification：The antibody was purified by immunogen affinity chromatography.

Specificity：Recognizes endogenous levels of PPAR gamma protein.

Clonality：Polyclonal

Form：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Dilution：WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)

Gene Symbol：PPARG

Alternative Names：NR1C3; Peroxisome proliferator-activated receptor gamma; PPAR-gamma; Nuclear receptor subfamily 1 group C member 3

Entrez Gene (Human)： 5468;

Entrez Gene (Mouse)： 19016;

Entrez Gene (Rat)： 25664;

SwissProt (Human)： P37231;

SwissProt (Mouse)： P37238;

SwissProt (Rat)： O88275;

Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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