General Information
| Short Description | Rabbit polyclonal anti-Phosphatidylinositol 3-kinase regulatory subunit alpha and Phosphatidylinositol 3-kinase regulatory subunit gamma (436-485 aa) for use in WB, IHC, IF and ELISA in Human, Mouse, Rat, Monkey and Chicken samples. Datasheet include |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat/Monkey/Chicken |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300IF 1:200-1:1000ELISA 1:20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | |
| Gene ID | |
| Uniprot ID | |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human PI3-kinase p85-alpha/gamma at the amino acid range 436-485 |
| Immunogen Region | 436-485 aa |
| Specificity | PI 3-kinase p85 Alpha/Gamma Polyclonal Antibody detects endogenous levels of PI 3-kinase p85 Alpha/Gamma protein. |
Additional Info
| Post Translational Modifications | Polyubiquitinated in T-cells by CBLB.which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation. Phosphorylated. Tyrosine phosphorylated in response to signaling by FGFR1, FGFR2, FGFR3 and FGFR4. Phosphorylated by CSF1R. Phosphorylated by ERBB4. Phosphorylated on tyrosine residues by TEK/TIE2. Dephosphorylated by PTPRJ. Phosphorylated by PIK3CA at Ser-608.phosphorylation is stimulated by insulin and PDGF. The relevance of phosphorylation by PIK3CA is however unclear. Phosphorylated in response to KIT and KITLG/SCF. Phosphorylated by FGR. |
| Function | Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling. Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement. |
| Protein Name | Phosphatidylinositol 3-Kinase Regulatory Subunit AlphaPi3-Kinase Regulatory Subunit AlphaPi3k Regulatory Subunit AlphaPtdins-3-Kinase Regulatory Subunit AlphaPhosphatidylinositol 3-Kinase 85 Kda Regulatory Subunit AlphaPi3-Kinase Subunit P85-AlphaPtdins-3-Kinase Regulatory Subunit P85-Alpha |
| Database Links | |
| Alternative Antibody Names | Anti-Phosphatidylinositol 3-Kinase Regulatory Subunit Alpha antibodyAnti-Pi3-Kinase Regulatory Subunit Alpha antibodyAnti-Pi3k Regulatory Subunit Alpha antibodyAnti-Ptdins-3-Kinase Regulatory Subunit Alpha antibodyAnti-Phosphatidylinositol 3-Kinase 85 Kda Regulatory Subunit Alpha antibodyAnti-Pi3-Kinase Subunit P85-Alpha antibodyAnti-Ptdins-3-Kinase Regulatory Subunit P85-Alpha antibodyAnti-PIK3R1 antibodyAnti-GRB1 antibody |
Information sourced from Uniprot.org